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PsbX | small subunit X of PSII

345 €

AS05 066  |  clonality: polyclonal  |  host: rabbit  | reactivity:Arabidopsis thaliana

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Item No:
AS05 066

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product information
Background

Plant PsbX is a nucelar encoded small 4 kDa protein associated with Photosystem II and found in all classes of oxygenic organisms.

Immunogen

KLH-conjugated synthetic peptide dervied from Arabidopsis thaliana PsbX sequence At2g06520

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 200 ĩl
Reconstitution For reconstitution add 200 ĩl of sterile water
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

PSII available antibodies against Photosystem II proteins

Plant protein extraction buffer

Secondary antibodies

Additional information

PsbX is a small (4 kDa) and very hydrophobic subunit of PSII. Immunoblots from SDS-gels (especially with high loading) may show higher molecular weight signals from PsbX not fully detached from other subunits of PSII. The use of 15% SDS-PAGE gels with 2-4 M urea is recommended when working with this antibody.

application information
Recommended dilution

1: 2500 with standard ECL on 1 µg of chlorophyll/lane (WB)

Expected | apparent MW

4 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

di and monocots including Oryza sativa and Zea mays

Not reactive in

Cyanobacteria

Additional information

For an image of antibody detection in a western blot application see Garcia-Cerdan et al. (2008).

Selected references

Garcia-Cerdan et al. (2008). Antisense inhibition of the PsbX protein affects PSII integrity in the higher plant Arabidopsis thaliana. Plant Cell Physiol 50: 191-202



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