PSII (Photosystem II)
PSI/PSII ratio can be estimated once a quantitative immunoblot for PsaC (AS10 939) and for either PsbA (AS05 084) or PsbD (AS06 146), is performed in 3-4 replicates. Ratio calculation is done on a sample by sample basis. These ratios are based on the subunits present, whether they are in the assembled photosystem or not. Under some circumstances (eg. high light), there are biological reasons that the subunit numbers do not necessarily match the functional photosystems.
Literature
Brown et al. (2008). Flux capacities and acclimation costs in Trichodesmium from the Gulf of Mexico. Marine Biology 154 (3): 413-422.
Levitan et al. (2010). Regulation of nitrogen metabolism in the marine diazotroph Trichodesmium IMS101 under varying temperatures and atmospheric CO2 concentrations. Environmental Microbiology 12(7): 1899-1912.
Thamatrakoln et al. (2013). A ‘Death-Specific Protein’ in a marine diatom regulates photosynthetic responses to iron and light availability. Proceedings of the National Academy of Science 110(50): 20123-20128 Li G,
Li et al. (2014). The Nitrogen Costs of Photosynthesis in a Diatom under Current and Future pCO2. New Phytologist, 205:533-543.
Protocols and Technical Information • Support • Western Blot Video Tutorials
AS01 016 | Clonality: Polyclonal | Host: Hen | Reactivity: [global antibody] for higher plants, algae, cyanobacteria and dinoflagellates
Benefits of using this antibody
AS03 032 | Clonality: Polyclonal | Host: Hen | Reactivity: A. thaliana, A. sativa, B. napus, H. vulgare, O. sativa, P. sylvestris, P. tremula, S. oleracea
AS04 038 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, Physcomitrella patens, algae, cyanobacteria, diatoms
Benefits of using this antibody
This discounted antibody set contains the following high titer, well published antibodies and is suitable for Western blot using samples from a wide range of species making. This product suitable for teaching labs or mutant screening.
Benefits of using this antibody set
AS05 059 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, N. tabacum, P. sativum, S. oleracea, T. aestivum
AS05 060 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Nicotiana tabacum, Spinacia oleracea
AS05 066 | Clonality: Polyclonal | Host: Rabbit | Reactivity:Arabidopsis thaliana
The set is suitable for Western blot using samples from a wide range of species making this product suitable for teaching labs or mutant screening.
Benefits of using this antibody set
AS05 084 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms | cellular [compartment marker] of thylakoid membrane
Benefits of using this antibody
AS05 084A | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms compartment marker of thylakoid membrane
Benefits of using this antibody
AS05 084A-ALP | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms compartment marker of thylakoid membrane
Benefits of using this antibody
AS05 084A-FITC | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms compartment marker of thylakoid membrane
Benefits of using this antibody
AS05 084A-HRP | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms compartment marker of thylakoid membrane
Benefits of using this antibody
AS05 084B | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for higher plants, algae, liverwort, cyanobacteria, diatoms
compartment marker of thylakoid membrane
Benefits of using this antibody
AS05 092 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, N. tabacum, P. sativum, Z. mays
AS06 111 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Cyanobacteria
AS06 112 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, H. vulgare, N.tabacum, O. sativa, S. oleracea | cellular [compartment marker] of thylakoid membrane
AS06 113 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, P.sativum, T. aestivum
AS06 114 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
AS06 142-16 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, P. abies, T. aestivum, Z. mays
AS06 142-23 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, Ch. reinhardtii, P. banksiana. , S.oleracea, T. aestivum
AS06 142-33 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, C. reinhardtii, C. vulgaris, C. sp. DT, E. crus-galli, F. arundinacea cv. Kord, H. pluvialis, H. spontaneum, H. vulgare, N. benthamiana, P. abies, P. banksiana, P. sativum, P. miliaceum, S. oleracea, S. tuberosum cultivar Taedong Valley, Synechococcus sp. PCC 7942 and PCC6803, T. salsuginea,
T.aestivum
compartment marker of thylakoid lumen Replaced by AS05 092
AS06 146 | Clonality: Polyclonal | Host: Rabbit | Reactivity: [global antibody] for A. thaliana, Anabaena 7120, D. brightwellii, H. vulgare, C.reinhardtii, C. zofingiensis, L. corniculatus, N. tabacum, O. sativa, P. sativum, P. vulgaris, P. tricornutum, T. pratense, S. alba, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803, T. guillardii, T. pseudonana, Triticale, U. prolifera, Z. mays
Benefits of using this antibody
AS06 157 | Clonality: Polyclonal | Host: Rabbit | Reactivity: plants including A. balsamea, A.thaliana, H.vulgare, P.strobus, S.oleracea, Synechococcus sp. PCC 7942, Z.mays
AS06 158 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A.thaliana, H.vulgare
AS06 167 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, H. vulgare, N. tabacum, P.abies, P. sativum, P. patens
AS06 171 | Clonality: | Polyclonal | Host: Rabbit | Arabidopsis thaliana