PYR1 | Abscisic acid receptor RCAR11
AS13 2634 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana
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1 : 10 000 with standard ECL (WB)
|Expected | apparent MW||
|Confirmed reactivity||Arabidopsis thaliana|
|Predicted reactivity||Brassica sp.|
|Not reactive in||no confirmed exceptions from predicted reactivity are currently known|
to be added when available
to be added when available, antibody released in November 2014.
150 µg of total protein from Arabidopsis thaliana (col-0) extracted with 50mM Tris, 150mM NaCl, 05%Triton X-100, 2mM DTT, 1mM PMSF, protease inhibitor were separated on 10 % SDS-PAGE and blotted 2h to PVDF using semi-dry. Blots were blocked with 1xTBST with nonfat milk 5% for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 for 1h at RT with agitation. The antibody solution was decanted and the blot washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:50 000 in for 1h at RT with agitation. The blot was washed for 15 min and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 5 min. Longer exposure time for wt samples is necessary.
Courtesy, Dr. Joanna Kufel, Institute of Genetics and Biotechnology, Warsaw University
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