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RH3 | RNA helicase (chloroplastic)
AS13 2714 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, Z. mays
- Product Info
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Immunogen: recombinant RH3 from Zea mays, amino acids 531-691, conserved in Zea mays RH3A (GRMZM2G415491_P01) and RH3B (GRMZM2G163072_P01) and Arabidopsis thaliana At5g26742
Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Immunoprecipitation (IP), Western blot (WB) Recommended dilution: 1 : 1000 (WB) Expected | apparent MW: 75 kDa
- Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Zea mays Predicted reactivity: Panicum italicum, Oryza brachyantha, Oryza sativa
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Application example
5 µg of total seedling leaf protein or from the indicated subcellular fractions from Zea mays were separated on 12% SDS-PAGE and blotted to nitrocellulose. Blots were blocked with 4% milk for 1h at room temperature (RT) with agitation. THe blot was incubated in the primary antibody at a dilution of 1: 1000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (goat anti-rabbit IgG conjugated to horse radish peroxidase) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above, and imaged by ECL using a LiCor digital imager with a 1 minute exposure.
Courtesy of Dr. Alice Barkan, University of Oregon, USA - Background
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Background: RH3 (RNA helicase) is involved in RNA synthesis, modification, cleavage and degradation. RH3 is the most abundant plastid DEAD box RNA helicase in maize and Arabidopsis thaliana.
- Product Citations
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Selected references: Bach-Pages et al. (2020). Discovering the RNA-Binding Proteome of Plant Leaves With an Improved RNA Interactome Capture Method. Biomolecules. 2020 Apr 24;10(4):E661. doi: 10.3390/biom10040661.
Asakura et al. (2012). Chloroplast RH3 DEAD box RNA helicases in maize and Arabidopsis function in splicing of specific group II introns and affect chloroplast ribosome biogenesis. Plant Physiol. 2012 Jul;159(3):961-74. doi: 10.1104/pp.112.197525. Epub 2012 May 10.
- Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts - Reviews:
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J ZHU | 2023-11-13It works with Arabidopsis RH3
Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
AS09 607 | Clonality: Polyclonal Host: Goat Reactivity: Rabbit IgG (H&L)
This product can be purchased in 3 different volumes:
AS16 ECL-S-10, 10 ml. Trial size limited to one per customer
AS16 ECL-S-100, 100 ml
AS16 ECL-S-1L, 1L
Choose the appropriate volume in the drop down menu to the right