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RH3 | RNA helicase, chloroplastic

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AS13 2714 | clonality: polyclonal  |  host: rabbit  |  reactivity: A. thaliana, Z. mays

PRODUCT INFORMATION IN PDF

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AS13 2714

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product information
background  

RH3 (RNA helicase) is involved in RNA synthesis, modification, cleavage and degradation. RH3 is the most abundant plastid DEAD box RNA helicase in maize and Arabidopsis thaliana.

immunogen  

recombinant RH3 from Zea mays, amino acids 531-691, conserved in Zea mays RH3A (GRMZM2G415491_P01) and RH3B (GRMZM2G163072_P01)

antibody format  

rabbit

polyclonal

serum

lyophilized

quantity  

50 µl

for reconstitution add 50 µl, of sterile water.

storage  

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications  

western blot (WB),  immunoprecipitation (IP)

related products   antibodies to RNA metabolism
additional information  
application information
recommended dilution  

1 : 1000 with standard ECL (WB)

expected | apparent MW  

kDa

confirmed reactivity   Arabidopsis thaliana, Zea mays
predicted reactivity  

Panicum italicum, Oryza brachyantha, Oryza sativa

not reactive in  

no confirmed exceptions from predicted reactivity are currently known

additional information  
selected references  

Asakura et al. (2012). Chloroplast RH3 DEAD box RNA helicases in maize and Arabidopsis function in splicing of specific group II introns and affect chloroplast ribosome biogenesis. Plant Physiol. 2012 Jul;159(3):961-74. doi: 10.1104/pp.112.197525. Epub 2012 May 10. 


Application example

 

5 µg of total seedling leaf protein or from the indicated subcellular fractions from Zea mays were separated on 12% SDS-PAGE and blotted to nitrocellulose. Blots were blocked with 4% milk for 1h at room temperature (RT) with agitation. THe blot was incubated in the primary antibody at a dilution of 1: 1000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (BioRad goat anti-rabbit IgG conjugated to horse radish peroxidase) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above, and imaged by ECL using a LiCor digital imager with a 1 minute exposure.

Courtesy of Dr. Alice Barkan, University of Oregon, USA


||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at  support@agrisera.com

Do you have a matching secondary antibody?

AS09 602 | Goat anti-rabbit IgG (H&L), HRP conjugated

AS09 607 | Goat anti-rabbit IgG (H&L), ALP conjugated 

AS09 603 | Goat anti-chicken IgY (H&L), HRP conjugated

AS09 606 | Goat anti-chicken IgY (H&L), ALP conjugated

For a complete list of Agrisera secondary antibodies


Protein extaction buffer:

AS08 300 | Plant and Algal Protein Extraction Buffer (PEB)
An extraction buffer for quantitative isolation of total soluble/membrane protein from various tissues, optimized for subsequent western blot detection.