RH3 | RNA helicase, chloroplastic
AS13 2714 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, Z. mays
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1 : 1000 with standard ECL (WB)
|Expected | apparent MW||
|Confirmed reactivity||Arabidopsis thaliana, Zea mays|
Panicum italicum, Oryza brachyantha, Oryza sativa
|Not reactive in||
no confirmed exceptions from predicted reactivity are currently known
Asakura et al. (2012). Chloroplast RH3 DEAD box RNA helicases in maize and Arabidopsis function in splicing of specific group II introns and affect chloroplast ribosome biogenesis. Plant Physiol. 2012 Jul;159(3):961-74. doi: 10.1104/pp.112.197525. Epub 2012 May 10.
5 µg of total seedling leaf protein or from the indicated subcellular fractions from Zea mays were separated on 12% SDS-PAGE and blotted to nitrocellulose. Blots were blocked with 4% milk for 1h at room temperature (RT) with agitation. THe blot was incubated in the primary antibody at a dilution of 1: 1000 for 2h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (BioRad goat anti-rabbit IgG conjugated to horse radish peroxidase) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above, and imaged by ECL using a LiCor digital imager with a 1 minute exposure.
Courtesy of Dr. Alice Barkan, University of Oregon, USA
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