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Rhodanese/cell cycle control phosphatase superfamily protein

265 €
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AS15 2900 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

PRODUCT INFORMATION IN PDF

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Item No:
AS15 2900

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product information
Background

Rhodanese/cell cycle control phosphatase superfamily protein is localized in chloroplasts. 

Immunogen

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana ATCaS sequence UniProt: F4J9G2, TAIR:AT3G59780

Host Rabbit
Clonality Polyclonal
Clone
Purity

Serum

Format

Lyophilized

Quantity

50 µl

Reconstitution

For reconstitution add 50 µl of sterile water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications

Western blot (WB)

Related products

Antibodies to proteins involved in photosynthesis

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1:1000 (WB)

Expected | apparent MW

73.9 | 69 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity

Brassica napus, Gossypium arboreum 

Not reactive in no confirmed exceptions from predicted reactivity known in the moment
Additional information
Selected references

To be added when available, antibody released in October 2016.


Application example

western blot using anti-ATCasLike antibodies

10, 15, 20 µg of total protein from Arabidopsis thaliana chloroplast and thylakoids were separated on 12 % SDS-PAGE and blotted 1h to nitrocellulose. Blots were blocked with 10% Non fat dairy milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was washed twice, then washed three times for 15 minutes in TBS-T at RT weith agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 in TBST for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturers instructions. Exposure time was 1 minute.

Courtesy of Dr. Rikard Fristedt VU University Amsterdam, The Netherlands


||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com