Plant/Algal cell antibodies / Carbohydrates

product information

This antibody specifically cross-reacts against xylose residues bound to the protein N-glycans in beta1,2. This residue is characterisitc of the plant protein N-glycans and is absent in protein N-glycans from animals. This residue is added in the Golgi apparatus.

xylose residues bound to the N-glycan in beta 1,2

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS07 268 | anti-fucose rabbit antibody

beta (1,2) xylose is present exclusively in plant N-glycans so antibodies against this sugar moiety should not cross-react with any mammal glycoprotein.

application information

2 ug/10 ml incubation buffer with standard ECL (WB)

10-100 for various glycoproteins

higher plants

higher plants

no confirmed exceptions from predicted reactivity known in the moment

negative control:

Fetuin, a glycoprotein containing fucose linked in alpha 1.6 and no xylose, Sigma, product number  F3385

positive control:

Type II - horseradish peroxidase which contains β1.2 Xylose and α1.3 fucose, Sigma, product number P8250

Paulus et al. (2011). Silencing β1,2-xylosyltransferase in transgenic tomato fruits reveals xylose as constitutive component of Ige-binding epitopes. Frontiers in Plant Biotech. September 29.

Baiet et all. (2010). N-glycans of Phaeodactylum tricornutum diatom and functional characterization of its N-acetylglucosaminyltransferase I enzyme. J Biol. Chem. Dec 17.

application example

Total cell extract from Arabidopsis thaliana wild type (1) and cell extracts from different mutants defective in the complex N-glycan maturation pathway  (2-5) (data not published yet).

Primary antibody has been used at 2 µg/10 ml of incubation buffer. Detection has been done using ECL.