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14.3.3C | 14.3.3-like protein C

356 €

AS08 317  |  clonality: polyclonal  |  host: rabbit  |  reactivity: Hordeum vulgare

PRODUCT INFORMATION IN PDF

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Item No:
AS08 317

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product information
Background

14.3.3’s are 30KDa proteins involved in protein interactions with target proteins containing phosphorylated target sites. Functions of 14.3.3’s include acting as adaptors or scaffolds, stimulating protein-protein interaction, altering target protein activity, causing conformational changes of target proteins, regulating subcellular localisation and also facilitating transport (for example nuclear import/export and transport in the endomembrane system). A variety of apparently unrelated biological activities, including a role in development and signal transduction have been ascribed to the 14.3.3 family. To date, five barley 14.3.3 homologues have been identified and characterised and named 14.3.3A through E.

Immunogen

synthetic peptide specific to the barley C isoform, conjugated to KLH

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB), ELISA (ELISA)
Related products AS12 2119 | 14-3-3 GRF | General regulatory element, rabbit antibody to all GRF isoforms with broad species reactivity

matching secondary antibody for chemiluminescence detection
Additional information
application information
Recommended dilution

1: 2000 with standard ECL (WB), 1: 15 000 (ELISA)

Expected | apparent MW

30 kDa

Confirmed reactivity

Hordeum vulgare

Predicted reactivity

Horderum vulgare

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

specific for barley 14.3.3C isoform, when tested against 14.3.3A, B and C isoforms

Selected references

to be added when available


application example

 

 
 
SDS-PAGE gel and duplicate western blot of 1 - Hv14.3.3A; 2 - Hv14.3.3B and 3 - Hv14.3.3C, showing anti Hv14.3.3C antibody recognition only Hv14.3.3C protein band.
AS08 317 western.jpg

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