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aadA1 | Aminoglycoside adenyltransferase (chloroplast transformation marker)

345 €

AS09 580  clonality: polyclonal  |  host: rabbit  |  reactivity: Nicotiana tabacum, Chlamydomonas reinhardtii

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Item No:
AS09 580

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product information
Background

aadA - aminoglycoside 3"adenyltransferase is an enzyme with nucleotidyltransferase activity. Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection. 

Immunogen

KLH-conjugated peptide derived from known aadA1 sequences

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 200 µg
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
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Additional information
application information
Recommended dilution 1 : 3 000 (WB)
Expected | apparent MW

30 | 30 kDa

Confirmed reactivity aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii
Predicted reactivity

Acinetobacter baumannii, Enterobacter agglomerans, Klebsiella pneumoniae, Klebsiella oxytoca, Kluyvera ascorbata, Pseudomonas aeruginosa,  Salmonella enterica subsp. enterica serovar Worthington, Staphylococcus aureus, Serratia sp., Shigella flexneri

Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references

to be added when available, antibody available in July 2010


application example

western blot using aadA1 antibodies

Chlamydomonas reinhardtii cells (1,2) (corresponding to 10 ug  of chlorophyll) and soluble extracts of Nicotiana tabaccum leaves (3,4) were separated on a  15% acrylamide gel containing 8M urea, transferred on nitrocellulose membrane, incubated with anti-aad1 antibodies in 1: 5000 dilution for 1 h in RT, followed by washes and incubation with a secondary anti-rabbuit IgG antibody in  1: 10 000 dilution) and revealed by ECL (GE Healthcare; 3 min exposure). Tested Chlamydomonas reinhardtii and Nicotiana tabaccum strains expressed (+) or not (-) the aadA cassette.
AadA protein in Chlamydomonas reinhardtii migrates more slowly than its Nicotiana tabaccum counterpart as it also includes the 27 first residues of the atpA coding sequence.

Courtesy of Drs Y.Choquet, K. Wostrikoff and D. Drapier, CNRS, France

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com