BIK1 | Botrytis-induced kinase 1

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AS16 4030 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana


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Item No:
AS16 4030

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product information
Background BIK1 (Botrytis-induced kinase 1) belongs to the protein kinase superfamily. BIK1 is a crucial component of host response signaling required to activate the resistance responses to Botrytis and A. brassicicola infection.

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BIK1 sequence, UniProt: O48814, TAIR: AT2G39660

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized in PBS pH 7.4
Quantity 50 µg
Reconstitution For reconstitution add 50 µl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Western blot (WB)
Related products

antibodies to proteins involved in SA response

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 3000 (WB)

Expected | apparent MW 44 kDa
Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Noccaea caerulescens
Not reactive in

Nicotiana benthamiana

Additional information
Selected references

To be added when available, antibody released in November 2017.

Application example

western blot using anti-BIK1 antibodies

Total protein from 7-day old Arabidopsis thaliana seedlings Col-0 (wild-type) and bik1 (null mutant) (Lu et al, 2009) were extracted with 50mM HEPES-KOH buffer containing 250 mM sucrose, 5% glycerol, 50 mM NaPP, 1 mM NaMo, 25 mM NaF, 10mM EDTA, 0.5% PVP, 3mM DTT, 1mM PMSF, 10uM Leupeptin & 10nM Calyculin, and then fractionated by ultracentrifugation at 100,000 x gravity for 30 min at 4°C into soluble (S100) and microsomal (P100) proteins as described in LaMontagne et al. (2016). 30 µg proteins of Total, S100, and P100 fractions were denatured at 65°C for 5 min, separated on a 10 % SDS-PAGE and blotted 1h to nitrocellulose using tank transfer. Blots were blocked with 1x PBS (from Fisher Scientific BP665-1) + 0.1 %Tween 20 (PBS-T) + 5% milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1000 overnight at 4°C with agitation in 1x PBS-T + 5% milk. The antibody solution was decanted, and the blot was rinsed briefly once, then washed three times for 7 min in 1x PBS-T at RT with agitation. Blot was incubated with secondary antibody (Goat anti Rabbit IgG (H&L) –HRP conjugated from Agrisera (AS09 602) diluted to 1:10 000 in 1x PBS-T + 5% milk for 2 hr at RT with agitation. The blot was washed as above and developed for 5 min with Amersham ECL (RPN2106). Exposure time was 6 min.

Courtesy of Gayani Ekanayake & Dr. Antje Heese Division of Biochemistry, Interdisciplinary Plant Group (IPG) - University of Missouri; Columbia, MO, USA

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