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FMR | Fumarate reductase

333 €

AS12 2618  | clonality: polyclonal  |  host: rabbit  |  reactivity: Chlamydomonas reinhardtii

 

PRODUCT INFORMATION IN PDF

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Item No:
AS12 2618

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product information
Background

FMR (fumarate reductase) is an enzyme that catalyze the final steps of succinate synthesis.

Immunogen KLH-conjugated synthetic peptide derived Chlamydomonas reinhardtii FMR protein seqeunce, protein ID 145357.
Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 200 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS12 1617 | anti-MME4 | malic enzyme

Algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 1000 with alkaline phosphatase (WB)

Expected | apparent MW

57 kDa

Confirmed reactivity

Chlamydomonas reinhardtii

Predicted reactivity Leishmania mexicana, Naegleria gruberi (Amoeba)
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information

to be added when available

Selected references Subramanian et al. (2014). Profiling Chlamydomonas Metabolism under Dark, Anoxic H 2 ‐Producing Conditions Using a Combined Proteomic, Transcriptomic, and Metabolomic Approach. J Proteome Res. 2014 Oct 21.

application example


western blot using anti-FMR antibody

25 µg of total protein from Chlamydomonas reinhardtii, oxic conditions (A), dark anoxia (B) were separated on 4-15 % SDS-PAGE and blotted 1h to PVDF. Blotting was done using SNAP-ID kit: incubation in blocking buffer for 1 min., following incubation in a primary antibody at a dilution of 1: 1 000 for 20 min, wash three times with wash buffer TBS-T, followed by incubation ina secondary antibody at a dilution of 1: 5000, for 20 min. and three times wash in TBS-T.  The blot was washed   and developed with AP color development reagent (AP conjugate substrate kit from Bio-rad) according to the manufacturer's instructions.

Courtesy of Dr. Alexandra Dubini


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