GID2 | F-BOX protein GID2 (SLEEPY 1)
AS13 2638 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana
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|Recommended dilution||1 : 5000 (WB)|
|Expected | apparent MW||
17.4 | 18 kDa (Arabidopsis)
|Confirmed reactivity||Arabidopsis thaliana|
|Predicted reactivity||Brassica napus, Medicago truncatula, Glycine max, Ricinus communis, Populus trichocarpa, Vitis vinifera|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
to be added when available, antibody released in April 2014.
15µg of total protein from inflorescence of Col0, spy mutant, and 35S-SPY extracted with extraction buffer (DTT 100uM, Tris pH 6,8 67,5mM, Urea 4M, SDS 3%,glycerol 3% and bromophenol 0,1%) were separated on 10% SDS-PAGE and blotted 1h to PVDF. Blots were blocked with milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody diluted to 1:10 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 2 min with ECL (Luminata Forte) according to the manufacturer's instructions. Exposure time was 30 seconds.
Courtesy Dr. Patrick Achard, CNRS, France
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