GID2 | F-BOX protein GID2 (SLEEPY 1)
AS13 2638 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana
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1 : 5000 (WB)
|Expected | apparent MW||
17.4 | 18 kDa (Arabidopsis)
|Predicted reactivity||Brassica napus, Medicago truncatula, Glycine max, Ricinus communis, Populus trichocarpa, Vitis vinifera|
|Not reactive in||
no confirmed exceptions from predicted reactivity are currently known
to be added when available
to be added when available, antibody released in April 2014.
15µg of total protein from inflorescence of Col0, spy mutant, and 35S-SPY extracted with extraction buffer (DTT 100uM, Tris pH 6,8 67,5mM, Urea 4M, SDS 3%,glycerol 3% and bromophenol 0,1%) were separated on 10% SDS-PAGE and blotted 1h to PVDF. Blots were blocked with milk for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 overnight at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 3 times for 10 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody diluted to 1:10 000 in TBS-T for 1h at RT with agitation. The blot was washed as above and developed for 2 min with ECL (Luminata Forte) according to the manufacturer's instructions. Exposure time was 30 seconds.
Courtesy Dr. Patrick Achard, CNRS, France
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