ANTIBODY SHOP

Services
Name:
Phone:
E-mail:
Message

RGA | DELLA protein RGA

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each

AS11 1630 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana

PRODUCT INFORMATION IN PDF

Qty: 
Availability:
96 st
Delivery:
Shipping:
Item No:
AS11 1630

Info: Product suggestions Add review
product information
Background DELLA protein RGA is a putative transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. It is a member of VHIID/DELLA regulatory family and is the most sensitive to GA application, compare to other DELLA proteins.Involved in fruit and flower development.  Synonymes: GAI-related sequence, GRAS family protein 10, AtGRAS-10, Repressor on the ga1-3 mutant, Restoration of growth on ammonia protein 1.
Immunogen

KLH-conjugated peptide chosen from RGA of Arabidopsis thaliana Q9SLH3, At2g01570

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS11 1631 | GAI | DELLA protein GAI, rabbit antibody
AS06 194 | GA3 | gibberellic acid, rat antibody
AS12 1870
| PIF3 | Phytochrome interacting factor 3
AS12 1860 | PIF4 | PHYTOCHROME INTERACTING FACTOR 4
AS11 1802 | RGA-like protein | DELLA protein RGL1
AS11 1803
| RGA-like protein 2 | DELLA protein RGL2

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 1000 with standard ECL (WB)

Expected | apparent MW

64 | 64 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity only Arabidopsis thaliana
Not reactive in

Triticum aestivum

Additional information

RGA protein is prone to degradation therefore caution has to be taken during protein extraction.

Selected references Shahnejat-Bushehri et al. (2016). Arabidopsis NAC transcription factor JUB1 regulates GA/BR metabolism and signalling. NATURE PLANTS 2: Article number: 16013, 2016.
Crocco et al. (2015). The transcriptional regulator BBX24 impairs DELLA activity to promote shade avoidance in Arabidopsis thaliana. Nat Commun. 2015 Feb 6;6:6202. doi: 10.1038/ncomms7202.
Leone et al. (2014). To grow or defend? Low red : far-red ratios reduce jasmonate sensitivity in Arabidopsis seedlings by promoting DELLA degradation and increasing JAZ10 stability. New Phytol. 2014 Oct;204(2):355-67. doi: 10.1111/nph.12971. Epub 2014 Aug 7.

application example

western blot using anti-RGA antibodies

40 µg of total protein from 5-d-old dark-grown Arabidopsis thaliana seedlings extracted with 50 mM Tris-HCl pH 7.5,, 10% glycerol, 150 mM NaCl, 0.1% NP-40, 1 mM PMSF, and 1x protease inhibitor cocktail (Roche) were separated on 4-20 % SDS-PAGE and blotted 1h to PVDF. m: mock, P: paclobutyrazol, P+G: PAC+GAs. Blots were blocked with 2% blocking reagent (GE Healthcare) in TBS-T for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h at RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602 ) diluted to 1:10 000 in for 1h at RT with agitation. The blot was washed as above and developed for 5 min with Femto reagent (Pierce) according to the manufacturers instructions. Exposure time was 20 seconds in a LAS-3000 Imager (Fuji).

Courtesy of Dr. David Alabadi, IBMCP (CSIC-UPV), Valencia, Spain

westrn blot using anti-RGA antibodies


Arabidopsis thaliana seedlings were ground in liquid nitrogen (100 µl of 2.5 x Laemli for 80-120 mg of homogenized material) and boiled in 2,5x Laemmli Buffer (WITH 60 Mm DTT final concentration) otherwise RGA protein will degrade. Plants were grown on 1/ MS for 15 days and were treated with 1 µM GA for 2 hours (GA+) or without hormone (GA-). Total protein extracts were denatured  for 2 min. at 95°C and were separated on 10% SDS-PAGE and blotted overnight to PVDF using tank transfer. Blots were blocked for 1.5 h with TBS-T containing 5% low fat milk for 2h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 1 000 for 1h 30min at RT with agitation. The antibody solution was decanted and the blot was washed 5 x 10min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:10 000 for 1h 30 min at RT with agitation. The blot was washed 6 x 10min in TBS-T at RT with agitation and developed for 5 min with ECL according to the manufacturer's instructions. Exposure time was 5 seconds with West Femto (Pierce). 

Courtesy of Kamila Jaronczyk, IBB, Warsaw, Poland

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com