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HPR | Hydroxypyruvate reductase (peroxisomal matrix marker)

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AS11 1797 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana

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AS11 1797

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product information
Background

Hydroxypyruvate reductase (HPR) belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family (oxidoreductases) and is involved in glycine, serine and theronine and glyoxylate and dicarboxylate metabolism. Synonymes: HPR, beta-hydroxypyruvate reductase, NADH:hydroxypyruvate reductase, D-glycerate dehydrogenase.

Immunogen

KLH-conjugated synthetic peptide derived from known plant HRP sequences, including Arabidopsis thaliana Q9C9W5, At1g68010

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS08 372 | AtPex14p | peroxysomal marker, rabbit antibody

AS10 711 | DEG15 | endopeptidase, peroxisomal marker, rabbit antibody

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 10 000 (WB)
Expected | apparent MW

43 kDa

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Chlamydomonas reinhardtii, Chlorella sp. ,Cucumis sativus, Glycine max, Oryza sativa, Ricinus communis, Volvox
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references Farmer et al. (2013).Disrupting Autophagy Restores Peroxisome Function to an Arabidopsis lon2 Mutant and Reveals a Role for the LON2 Protease in Peroxisomal Matrix Protein Degradation. Plant Cell, Oct 31.

application example

western blot dection of plant HPR protein (plant peroxisomal marker)

5-day-old light-grownwild-type (Columbia) (1) and hpr1-1 null mutant (SALK_143584) Arabidopsis thaliana seedlings were ground with a pestle in a 1.5 mL tube on dry ice (about 12 seedlings or enough to give ~ 20 µL of tissue), and double volume (~ 40 µL) of NuPAGE 2x loading buffer (Invitrogen) was added. After centrifugation, 20 µL of the supernatant was transferred to a fresh tube with 2.1 µL 0.5 M DTT and boiled at 100 °C for 5 minutes. Samples were loaded onto a NuPAGE 10% Bis-Tris gel (Invitrogen) next to Cruz Markers (Santa Cruz Biotechnology). After electrophoresis, proteins were transferred for 30 minutes at 24 V to a Hybond ECL nitrocellulose membrane (Amersham Pharmacia Biotech) using NuPAGE transfer buffer (Invitrogen). The blot was blocked for 1 h at 4 °C in 8% non-fat dry milk in TBS-T (blocking buffer), and incubated overnight with agitation at 4˚C with primary antibodies, 1:10 000 diluted in blocking buffer. The antibody solution was decanted and the blot was rinsed twice for 5 min each at 4 °C in 8% non-fat dry milk in TBS-T with agitation. The blot was incubated with horseradish peroxidase-conjugated anti-rabbit secondary antibody (Santa Cruz Biotechnology) diluted to 1:5 000 in blocking buffer for 5 h at 4 °C with agitation. The blot was washed three times, for 5 min each, with TBS-T and developed with LumiGLO reagent (Cell Signaling Technology) according to the manufacturer's instructions. Exposure time was 3 seconds.

Courtesy of Sarah Bukhart and Bonnie Bartel, Rice University, USA


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