ANTIBODY SHOP

Services
Name:
Phone:
E-mail:
Message

KUA1 | MYB transcription factor

265 €
Buy 2 items of this product for 198 €/each
Buy 3 items of this product for 180 €/each

AS15 2989  | clonality: polyclonal  |  host: rabbit  |  reactivity: Arabidopsis thaliana

PRODUCT INFORMATION IN PDF

Qty: 
Availability:
30 st
Delivery:
Shipping:
Item No:
AS15 2989

Info: Product suggestions Add review
product information
Background KUA1 (MYB transcription factor) involved in regulation of hypocotyl elongation in response to darkness by enhancing auxin accumulation in a phytochrome-interacting factor (PIF) proteins-dependent manner. Promotes lateral roots formation and place a critical role in in developmentally regulated and dark-induced onset of leaf senescence by repressing the transcription of several genes involved in chloroplast function and responses to light and auxin. Promotes responses to auxin, abscisic acid (ABA), and ethylene. Alternative names: Myb-related protein H, AtMYBH, AtMYBS3, MYBS3-homolg protein, Protein KUODA1.
Immunogen

KLH-conjugated peptide derived from Arabidopsis thaliana KUA1 protein sequence, UniProt: Q9LVS0,
TAIR:AT5G47390 .The peptide used to elicit this antibody is also conserved in MYB1R1 of Triticum urartu UniProt:M7YW99 and MYBS3 of Oryza sativa subsp. japonica, UniProt: Q7XC57-2

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized 
Quantity 50 µl
Reconstitution

For reconstitution add 50 µl, of sterile water.

Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications

Western Blot (WB)

Related products AS16 3954 | Anti-PIF3 | Phytochrome interacting factor 3, goat antibodies
AS16 3157 | Anti-PIF4 | Phytochrome interacting factor 4, rabbit antibodies
AS16 3955 | Anti-PIF4 | Phytochrome interacting factor 4, goat antibodies
AS12 2112 | Anti-PIF5 | PHYTOCHROME INTERACTING FACTOR 5, rabbit antibodies
AS12 1867 | Anti-HY5 | Protein long hypocotyl 5, rabbit antibodies

Secondary antibodies

Additional information
application information
Recommended dilution 1: 5000 (WB)
Expected | apparent MW  39.6 kDa
Confirmed reactivity Arabidopsis thaliana, Beta vulgaris
Predicted reactivity Capsicum annuum, Cephalotus follicularis, Cicer arietinum, Glycine max, Glycine soja, Gossypium arboretum, Cucumis melo, Medicago truncatula, Nelumbo nucifera, Nicotiana tabacum, Nicotiana sylvestris, Theobroma cacao, Vigna radiata var. radiata
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information The antibody was used in tissue printing on German sugar beet and was found in vascular bundles.
Selected references

To be added when available, antibody released in August 2017.


Application example

Western blot using anti-KUA1 antibodies

10-20 µg of total protein from fresh Arabidopsis thaliana leaves was grinded and extracted in 10 mM Tris/HCL pH 7,5; 50 mM NaCl; 0,5 mM EDTA, 1% Triton X-100 supplemented with protease inhibitor cocktail (Sigma, P9599). After 15 minutes of incubation on ice the extracts were centrifuged at 6000g for 5 minutes at 4 degrees. The supernatant was mixed with 2X Laemli buffer and denatured at 96°C for 5 min. After cooling down, the samples were separated on 12 % SDS-PAGE gel and blotted to PVDF in 9 minutes using a Pierce G2 Fast Blotter in transfer buffer. Blots were blocked with 5% blocking solution (containing non-fat milk protein in TBS) for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 5000 overnight in 5% blocking solution. The antibody solution was decanted and the blot was rinsed briefly twice, then washed twice for 10 min with TBS-T and twice for 10 min in 5% blocking solution at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera AS09 602) diluted to 1:10 000 in 5% blocking solution for 90 minutes at RT with agitation. The blot was washed four times in TBS-T for 10 minutes as described above and developed using Luminata Crescendo Western HRP substrate (Millipore). Blots were imaged using a BioRad Chemidoc system using an exposure time of 300 seconds.

Courtesy Dr. Jozefus Schippers, University of Aachen, Germany

||| For other applications, usage on species other than stated above or any other questions, please use the LiveChat option or contact us at support@agrisera.com