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product information
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| background |
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Lhcb5 is one of the 3 minor highly conserved chlorophyll a/b-binding proteins associated with Photosystem II in plants and algae. As a part of the inner light-harvesting antenna it has been sugested to regulate (together with Lhcb4 and Lhcb6) the energy flow from the outer LHCII antenna to the PSII reaction center. |
| immunogen |
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BSA-conjugated synthetic peptide derived from Arabidosis thaliana Lhcb5 protein (At4g5340) sequence. This sequence is highly conserved in Lhcb5 proteins from monocots, dicots and conifers but only partial conserved in Physcomitrella patens and Chlamydomonas reinhardtii. |
| antibody format |
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rabbit |
polyclonal, |
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total IgG in PBS pH 7.4, |
lyophilized |
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| quantity |
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200 µl |
- for reconstitution add 200 µl of sterile water |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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western blot (WB); immunohistochemistry (IHC) |
| related products |
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LHC available antibodies against pigment-binding proteins PSII available antibodies against Photosystem II proteins |
| additional information |
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to be added when available |
application information
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| recommended dilution |
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1 : 1000, detected with standard ECL (WB) |
| expected | apparent MW |
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26 | 25-26 kDa for Arabidopsis thaliana |
| confirmed reactivity |
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Arabidopsis thaliana, Hordeum vulgare, Pisum sativum, Spinacia oleracea, Zea mays |
| predicted reactivity |
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angiosperms (monocots and dicots), gymnosperms |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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to be added when available |
| selected references |
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Zienkiewicz et al. (2011). High light stimulates Deg1-dependent cleavage of the minor LHCII antenna proteins CP26 and CP29 and the PsbS protein in Arabidopsis thaliana. Planta Aug 30. Andersson et al. (2001) Antisense inhibition of the photosynthetic antenna proteins CP29 and CP26: implications for the mechanism of protective energy dissipation. Plant Cell 13:1193-1204 |
application example
5 µg protein of (1) chloroplasts, (2) thylakoids, and (3) Photosystem I of Arabidopsis thaliana together with (4) BBY (Photosystem II) -particles from Pisum sativum were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose. Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-Lhcb5 (AS01 009, 1:1000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (90s, standard sensitivity).
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