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Patatin (affinity purified)

265 € / 132.50 €
Buy 2 items of this product for 99 €/each
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AS12 1842A   | clonality: polyclonal  |  host: rabbit  |  reactivity: Solanum tuberosum

PRODUCT INFORMATION IN PDF

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AS12 1842A

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product information
Background

Patatin is the most abundant protein in potato (Solanum tuberosum L.) tubers, about 60 % of a total protein. It serves as a storage protein. Mature tuber patatin variants are dimers of 40-42 kDa subunits without disulphide bridges.

Immunogen

KLH-conjugated synthetic peptide derived from a C-terminal part of 36 known isoforms of patatin from Solanum tuberosum including: Q3YJS9, Q3YJT0, Q42502, Q3YJT2

Host Rabbit
Clonality Polyclonal
Clone
Purity Affinity purified serum
Format Lyophilized in PBS pH 7.4
Quantity 50 ĩg
Reconstitution For reconstitution add 50 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB), immunoprecipitation (IP), immunolocalization (IL)
Related products

collection of antibodies to Solanum tuberosum

Plant protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution

1 : 2000 with standard ECL (WB), 1:100 (IL)

Expected | apparent MW

40-42 kDa

Confirmed reactivity Solanum tuberosum
Predicted reactivity Solanum tuberosum
Not reactive in

no confirmed exceptions from predicted reactivity are currently known

Additional information Imunolocalization analyses were performed on 10-mm-thick bud meristem sections cut by microtome following the protocol described by Paciorek et al. (2006).

The following antibodies and dilutions were used: anti- Patatin (1:100) (Agrisera, Sweden) and Alexa Fluor® 488 Goat Anti-Rabbit IgG (H+L) secondary antibody (1:100) (Molecular probes).

Immunofluorescence was observed with an IX81/FV500 confocal laser-scanning microscope (Olympus) equipped with a 488-nm argon ion laser and a 405-nm diode laser.
Selected references

to be added when available, antibody released in October 2012.



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