PhyA | Phytochrome A
AS07 220 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum
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|Recommended dilution||1 : 1000 (WB)|
|Expected | apparent MW||
|Confirmed reactivity||Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum
|Predicted reactivity||Brassica rapa, Lathyrus sativus, Fragaria ananassa, Glycine max, Lotus corniculatus, Medicago truncatula, Nicotiana benthamiana (PhyA1), Nicotiana tabacum, Pisum sativum, Populus balsamifera, Ricinus communis|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
|Additional information||Careful sample collection is adviced to assure the best results with this antibody.|
|Selected references||Pierto-Dapena et al. (2017). Seed-specific transcription factor HSFA9 links late embryogenesis and early photomorphogenesis. J Exp Bot. 2017 Feb 16. doi: 10.1093/jxb/erx020.
Paik et al. (2012). Phytochrome regulates translation of mRNA in the cytosol. PNAS 109 (4): 1335-1340.
10 µg of total protein extracted with PEB (AS08 300) from etiolated seedlings of Arabidopsis thaliana (1) was separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 2h (30V) to nitrocellulose. Filter was blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-PhyA (AS07 220, 1:1000, 19.5h) and secondary anti-rabbit (1:20000, 1h) antibody (HRP conjugated) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T (15, +5, +5, +5 min). All steps were performed at RT with agitation. Signal was detected with standard ECL (GE Healthcare) using a Fuji LAS-3000 CCD (300s, standard sensitivity).
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