Plant/Algal cell antibodies / Chlamydomonas reinhardtii

product information

Multi-subunit complex of cytb6/f is a crucial component for the photosynthetic electron transport chain of higher plants, green algae and cyanobacteria. This complex is catalyzing oxidation of quinols and the reduction the reduction of plastocyanin. This reaction allows to establish the proton force required for the ATP synthesis. Four major subunits build the complex: the petA gene product corresponding to a c-type cytochrome (cytf), the petB gene product corresponding to a b-type/c’-type cytochrome with three haems (cyt b6), the petD gene product (subunit IV, or suIV), and the petC gene product, corresponding to the Rieske/Iron/sulfur protein.

GST fusion to cytochrome f from Chlamydomonas reinhartii  P23577

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS07 231 | anti-cytochrome f (with reactivity to monocots)

AS08 306 | anti-cytochrome f  (with reactivity to cyanobacteria)

Collection of antibodies to Arabidopsis proteins

Collection of antibodies to Chlamydomonas proteins

contains 0.02 % sodium azide as preservative

application information

1:2000 with alkaline phosphatase, 1: 50 000 with ECL  (WB)

34 | 31-32 kDa

Arabidopsis thaliana, Hordeum vulgare, Physcomitrella patens, Chlamydomonas reinhardtii

Citrus x limon, green algae

no confirmed exceptions from predicted reactivity known in the moment

reaction with cyanobacteria: Synechocystis 6803 and Synechococcus 7942 possible to obtain on total cell extract when using antibody at 1: 500 and longer exposure time.

Lecier et al. (2011). Functional analysis of hydrogen photoproduction in respiratory-deficient mutants of Chlamydomonas reinhardtii. International Journal of Hydrogen Energy

Lang, E.G.E., S.J. Mueller, S.N.W. Hoernstein, J. Porankiewicz-Asplund, M. Vervliet-Scheebaum, R. Reski (2010). Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as basis for sub-cellular proteomics. Plant Cell Reports, DOI: 10.1007/s00299-010-0935-4. (open source)

Page et al.  (2004): A Homolog of Prokaryotic Thiol Disulfide Transporter CcdA Is Required for the Assembly of the Cytochrome b6f Complex in Arabidopsis Chloroplasts. J. Biol. Chem., Vol. 279, 32474-32482

application example

                                                       Chlamydomonas reinhardtii samples are lysed cells,  Arabidopsis thaliana samples are prepared from thylakoid membranes; Samples were boiled 1 min in 3%SDS,then 10ug of chlorophylls were loaded in each well of a 12% acrylamide gel.