PetC | Rieske iron-sulfur protein of Cyt b6/f complex
AS08 330 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, C. reinhardtii, E. crus-galli, Euglena sp., H. pluvialis, N. tabacum, P. miliaceum, P. sativum, Synechococcus PCC 7942, Thalassiosira guillardii, Z. mays
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|Recommended dilution||1 : 5000-1 : 10 000 (WB)|
|Expected | apparent MW||
|Confirmed reactivity||Arabidopsis thaliana, Chlamydomonas reinhardtii, Echinola crus-galli, Euglena sp., Haematococcus pluvialis, Nicotiana tabacum, Panicum miliaceum, Pisum sativum, Synechococcus PCC 7942, Synechocystis sp. PCC 6803, Thalassiosira guillardii, Zea mays|
Acetabularia acetabulum, Brachypodium distachyon, cyanobacteria, Calothrix sp. PCC 7507, Cicer arietinum, Crocosphaera watsonii, Cynodon dactylon, Gossypium raimondii , Hordeum vulgare, Lyngbya aestuarii,Microcystis aeruginosa, Nicotiana tabacum, Pisum sativum, Ricinus communis , Saccharum hybrid cultivar ROC22, Selaginella moellendorffii , Sorghum bicolor, Spinacia oleracea, Oryza sativa, Physcomitrella patens, Phormidesmis priestleyi, Populus trichocarpa, Sonneratia alba, Triticum aestivum, Zostera marina, Vitis vinifera
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
|Selected references||Zang et al. (2017). Characterization of the sulfur-formation (suf) genes in Synechocystis sp. PCC 6803 under photoautotrophic and heterotrophic growth conditions. Planta. 2017 Jul 14. doi: 10.1007/s00425-017-2738-0.
Nath et al. (2016). A Nitrogen-Fixing Subunit Essential for Accumulating 4Fe-4S-Containing Photosystem I Core Proteins. Plant Physiol. 2016 Dec;172(4):2459-2470. Epub 2016 Oct 26.
Zhang et al. (2016). A new paradigm for producing astaxanthin from the unicellular green alga Haematococcus pluvialis. Biotechnol Bioeng. 2016 Oct;113(10):2088-99. doi: 10.1002/bit.25976. Epub 2016 Mar 28.
Han et al. (2015). A nuclear-encoded chloroplast-targeted S1 RNA-binding domain protein affects chloroplast rRNA processing and is crucial for the normal growth of Arabidopsis thaliana. Plant J. 2015 Jul;83(2):277-89. doi: 10.1111/tpj.12889. Epub 2015 Jun 15.
Li et al. (2015). The nitrogen costs of photosynthesis in a diatom under current and future pCO2. New Phytol. 2015 Jan;205(2):533-43. doi: 10.1111/nph.13037. Epub 2014 Sep 25.
Wang et al. (2014). Induction of caspase-3-like activity in rice following release of cytochrome-f from the chloroplast and subsequent interaction with the ubiquitin-proteasome system. Sci Rep. 2014 Aug 8;4:5989. doi: 10.1038/srep05989.
Hojka et al. (2014). Inducible repression of nuclear-encoded subunits of the cytochrome b6f complex in tobacco reveals an extraordinarily long lifetime of the complex. Plant Physiol. 2014 Jun 24. pii: pp.114.243741.
5 µg of total protein from (1) Arabidopsis thaliana leaf extracted with Protein Extration Buffer, PEB (AS08 300), (2) Euglena sp. extracted with PEB, (3) Synechococcus elongatus whole cell extracted with PEB, were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).
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