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product information
|
| background |
|
Ferritin is the major iron storage protein complex in eukaryotic cells, consisting of 24 ferritin subunit polypeptides. In Chlamydomonas reinhardtii 2 isoforms, ferritin1 (Fer1) and ferritin2 (Fer2) are present. Both are plastid localized but Fer1 is quantitatively recovered in soluble extracts of cells while Fer2 is found in the particulate fraction. Partial purification of the Fer1 complex indicates that the two ferritins are associated in distinct complexes and do not coassemble. The ratio of Fer1 to Fer2 in iron-replete cells is 70:1 and indicates a more dominant role of Fer1 in iron homeostasis. |
| immunogen |
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KLH-conjugated synthetic peptide derived from Chlamydomonas reinhardtii ferritin1 protein sequence Q8LRU1 |
| antibody format |
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rabbit, |
polyclonal |
|
serum, |
lyophilized |
|
| quantity |
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200 µl |
- for reconstitution add 200 µl of sterile water. |
|
| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB) |
| related products |
|
AS06196 Fer2 | ferritin 2 Collection of antibodies to Chlamydomonas proteins |
| additional information |
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to be added when available |
application information
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| recommended dilution |
|
1:1000 with standard ECL (WB) |
| expected | apparent MW |
|
27 (with transitpeptide) | 25 for Chlamydomonas reinhardtii |
| confirmed reactivity |
|
Chlamydomonas reinhardtii |
| predicted reactivity |
|
Chlamydomonas reinhardtii |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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a picture with a western blot application sample can be obtained from the reference given below |
| selected references |
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Long et al. (2008) Genetics 179: 137-147 |
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
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