Plant/Algal cell antibodies / Compartment markers / ER marker

product information

BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER.  BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER). Alternative name: AtBP2

KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP2  Q39043. Chosen peptide is also conserved in Arabidopsis thaliana BiP1 protein.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS09 614 | BiP2 | lumenal-binding protein, 2 hen antibody

AS09 481 | BiP2 | lumenal-binding protein 2, rabbit antibody

antibodies to plant endomembrane system proteins

Method for plant ER isolation is available here.

application information

1: 2000 with standard ECL (WB)

73.5 | 80 kDa

dicots including: Arabidopsis thaliana, Spinacia oleracea, monocots including: Hordeum vulgare, Zea mays

dicots including: Nicotiana tabacum, Spinacia oleracea, monocots: Hordeum vulgare, Oryza sativa, Zea mays, trees: Picea sitchensis, Populus trichocarpa, moss: Physcomitrella patens

no confirmed exceptions from predicted reactivity known in the moment

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Antibody has a reduced reactivity to monocots in western blot.

to be added when available, antibody released in September 2010

application example

5 µg of total protein from A.thaliana (1), H. vulgare (2),  Z.mays (3),  S. oleracea (4), extracted with Agrisera PEB extraction buffer (AS08 300) were separated on  4-12% SDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in  for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-goat IgG horse radish peroxidase conjugated, from  Agrisera AS09 605) diluted to 1:50 000  for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL  detection reagent according to the manufacturers instructions.  Exposure time was 5 seconds.