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product information
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| background |
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Heat shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stress or insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70) is a highly conserved protein and a member of the family of molecular chaperones. Alternative names: HSP70.1, HSP70-1/HSP70-2, Heat shock 70 kDa protein 8 |
| immunogen |
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KLH-conjugated synthetic peptide conserved across all known sequences of HSP70 P08107 and HSC70 proteins P11142 |
| antibody format |
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rabbit; |
polyclonal; |
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affinity purified rabbit serum on peptide column, in PBS pH 7.4; |
lyophilized; |
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| quantity |
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100 µg |
- for reconstitution add 100 µl of sterile water |
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| storage |
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store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
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Western blot (WB), immunoprecipiation (IP) |
| related products |
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AS05 083 | anti-HSP70/HSC70 global antibody |
| additional information |
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for detection of plant and algal cytoplasmic hsp70 we recommend following product: AS08 371 |
application information
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| recommended dilution |
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1: 1000 with standard ECL (WB), 1: 10 000 with ECL Advance (GE Healthcare), 1: 1000 (IP) |
| expected | apparent MW |
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70 kDa |
| confirmed reactivity |
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fish, mammals |
| predicted reactivity |
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vertebrates including bovine, hen, mouse, rat; insects including Drosophila melanogaster |
| not reactive in |
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no confirmed exceptions from predicted reactivity known in the moment |
| additional information |
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this antibody is not suitable for work with samples from higher plants |
| selected references |
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to be added when available |
||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menu bar or contact us at support@agrisera.com
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