Plant/Algal cell biology / Global antibodies

product information

Rieske Iron-Sulfur Protein (Q9ZR03)is located in chloroplast thylakoid membrane as a component of cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. Alternative names: Rieske iron-sulfur protein, RISP, ISP, plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein, proton gradient regulation protein 1

This is a recombinant protein standard, source: Synechocystis PCC 6803.

does not apply

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS07 230 | anti-PetC, Rieske iron-sulfur protein of Cyt b6/f complex

collection of antibodies to proteins involved in electron transfer

collection of other protein standards

Collection of global antibodies

global antibodies are raised against highly conserved amino acid sequences in the PetC protein. The PetC protein standard can therefore be used in combination with global anti-PetC antibodies to quantitate PetC from a wide range of species.

Quantitative western blot:  detailed method description.

application information

standard curve: 3 loads are recommended (0.5, 2 and 4μl).
For most applications a sample load of 0.2μg of chlorophyll will give a PsbA signal in this range.

positive control:a 2μl load per well is optimal for most chemiluminescent detection systems.

33 kDa (larger than native protein due to the addition of His-tag). In most gel systems, PetC protein migrates at 23 kDa

does not apply

does not apply

no confirmed exceptions from predicted reactivity known in the moment

Concentration: after adding 225  µl of milliQ water final concentration of the standard is 0.15 pmol/µl

Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50mM DTT.

This standard is stabilized and does not require heating before loading on the gel.

to be added when available. Standard released in May 2010