Plant/Algal cell biology / Global antibodies

product information

Photosystem I (PSI) of chloroplasts is a multisubunit membrane-protein complex that catalyzes the electron transfer from the reduced plastocyanin (or cytochrome c6) in the thylakoid lumen to the oxidized ferredoxin (or flavodoxin) in the chloroplast stroma. PsaB is a core protein of PSI complex. Synonymes: Photosystem I P700 chlorophyll a apoprotein A2, PSI-B

KLH-conjugated synthetic peptide derived from known PsaB sequences including Arabidopsis thaliana P56767

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS06 172 | anti-PsaA rabbit antibody

collection of antibodies to PSI proteins

to be added when available

application information

1 : 1000 with standard ECL (WB)

82.7 | 55-60 kDa

Arabidopsis thaliana, Hordeum vulgare, Chlamydomonas reinhardtii, Synechococcus sp. PCC7942

dicots including: Glycine max, Solanum tuberosum, monocots including: Triticum aestivum, Oryza sativa, Zea mays, trees: Pinus thunbergii, Populus trichocarpa, algae, cyanobacteria

no confirmed exceptions from predicted reactivity known in the moment

to be added when available

to be added when available. Antibody released in March 2010

application example

5 µg of total protein from (1) Arabidopsis thaliana leaf extract, (2) Synechococcus sp. PCC 7942 , (3) Hordeum vulgare leaf extract,  (4) Physcomitrella patens,  (5) Pisum sativum, (6) Zea may were extracted with Agrisera Protein Extraction Buffer PEB and separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to nitrocellulose OSMONICS. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Agrisera, AS09 602) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 30 seconds.