aadA1 | aminoglycoside adenyltransferase (chloroplast transformation marker)

320 €

AS09 580 | clonality: polyclonal | host: rabbit | reactivity: Nicotiana tabacum, Chlamydomonas reinhardtii

PRODUCT INFORMATION IN PDF

Info:

Product suggestions

Add review

product information

background

aadA - aminoglycoside 3"adenyltransferase is an enzyme with nucleotidyltransferase activity. Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection.

immunogen

KLH-conjugated peptide derived from known aadA1 sequences

antibody format

rabbit;

polyclonal;

affinity purified serum;

lyophilized

quantity

200 µg

- for reconstitution add 200 µl of sterile water

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

Western blot (WB)

related products

antibody collection for Arabidopsis thaliana

additional information

to be added when available

application information
recommended dilution		1: 3 000 with standard ECL (WB)
expected \| apparent MW		30 \| 30 kDa
confirmed reactivity		aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii
predicted reactivity		aadA cassette
not reactive in		no confirmed exceptions from predicted reactivity known in the moment
additional information		to be added when available
selected references		to be added when available, antibody available in July 2010

application example

Solubilized whole Chlamydomonas reinhardtii cells (1,2) (corresponding to 10 ug of chlorophyll) and soluble extracts of Nicotiana tabaccum leaves (3,4) were separated on a 15% acrylamide gel containing 8M urea, transferred on nitrocellulose membrane, incubated with anti-aad1 antibodies in 1: 5000 dilution for 1 h in RT, followed by washes and incubation with a secondary anti-rabbuit IgG antibody in 1: 10 000 dilution) and revealed by ECL (GE Healthcare; 3 min exposure). Tested Chlamydomonas reinhardtii and Nicotiana tabaccum strains expressed (+) or not (-) the aadA cassette.
AadA protein in Chlamydomonas reinhardtii migrates more slowly than its Nicotiana tabaccum counterpart as it also includes the 27 first residues of the atpA coding sequence.

Courtesy of Drs Y.Choquet, K. Wostrikoff and D. Drapier, CNRS, France

||| For applications or usage on species others than stated above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menu bar or contact us at support@agrisera.com