Plant/Algal cell antibodies / Photosynthesis / Other proteins

product information

aadA - aminoglycoside 3"adenyltransferase is an enzyme with nucleotidyltransferase activity. Plastid transformation in tobacco involves expression of aadA cassette, which confers resistance to spectinomycin and streptomycin and allows for transformat selection. 

KLH-conjugated peptide derived from known aadA1 sequences

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Western blot (WB)

antibody collection for Arabidopsis thaliana

to be added when available

application information

1: 3 000 with standard ECL (WB)

30 | 30 kDa

aadA casesette in Nicotiana tabacum, Chlamydomonas reinhardtii

aadA cassette

no confirmed exceptions from predicted reactivity known in the moment

to be added when available

to be added when available, antibody available in July 2010

application example 

Solubilized whole  Chlamydomonas reinhardtii cells (1,2) (corresponding to 10 ug  of chlorophyll) and soluble extracts of Nicotiana tabaccum leaves (3,4) were separated on a  15% acrylamide gel containing 8M urea, transferred on nitrocellulose membrane, incubated with anti-aad1 antibodies in 1: 5000 dilution for 1 h in RT, followed by washes and incubation with a secondary anti-rabbuit IgG antibody in  1: 10 000 dilution) and revealed by ECL (GE Healthcare; 3 min exposure). Tested Chlamydomonas reinhardtii and Nicotiana tabaccum strains expressed (+) or not (-) the aadA cassette.
AadA protein in Chlamydomonas reinhardtii migrates more slowly than its Nicotiana tabaccum counterpart as it also includes the 27 first residues of the atpA coding sequence.