|
product information
|
| background |
|
Photosystem I (PSI) of chloroplasts is a multisubunit membrane-protein complex that catalyzes the electron transfer from the reduced plastocyanin (or cytochrome c6) in the thylakoid lumen to the oxidized ferredoxin (or flavodoxin) in the chloroplast stroma. PsaN is necessary for docking plastocyanin to the PSI complex. PSI-N is the only subunit located entirely on the lumenal side of PSI. |
| immunogen |
|
KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana PsaN protein sequence At5g64040 |
| antibody format |
|
rabbit |
polyclonal |
|
affinity purified serum, in PBS pH 7.4, |
lyophilized |
|
| quantity |
|
200 µg, |
for reconstitution add 133 µl of sterile water. |
|
| storage |
|
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. |
| tested applications |
|
western blot (WB) |
| related products |
|
PSI antibody collection |
| additional information |
|
to be added when available |
application information
|
| recommended dilution |
|
1: 1000 with standard ECL (WB) |
| expected | apparent MW |
|
9.7 kDa |
| confirmed reactivity |
|
Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum |
| predicted reactivity |
|
dicots including Phaseolus vulgaris, Vitis vinifera, monocots including Oryza sativa, Zea mays, deciduous flowering plants including Populus balsamifera |
| not reactive in |
|
Chlamydomonas reinhardtii, Synechococcus so. 7942 |
| additional information |
|
to be added when available |
| selected references |
|
Hansson et al. (2007). Knock-out of the chloroplast encoded PSI-J Subunit of Photosystem I in Nicotiana tabacum: PSI-J is required for efficient electron transfer and stable accumulation of photosystem I. FEBS J. 274: 1734-1746. |
||| For applications or usage on species others than stated as confirmed above Agrisera offers a payment-after-testing option. To learn more about this or for any questions on this product, please use the LiveChat option in the left menue bar or contact us at support@agrisera.com
Save as PDF
|