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GS | Glutathione synthase, GSH-S

345 €

AS06 183  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Nicotiana tabacum, Solanum lycopersicum, Zea mays

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AS06 183

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product information
Background

Glutathione synthetase (GSH-S) catalyses the last step of glutathione (GSH) synthesis. GSH is synthesized in two ATP-dependent steps: in the first, gamma-glutamylcysteine (gamma-EC) is synthesized from L-glutamate and cysteine, catalyzed by gamma-glutamylcysteine synthetase (g-ECS); in the second step, catalysed by GSH-S, glycine is added to the C-terminal end of gamma-EC. GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development

Immunogen

KLH-conjugated synthetic peptide derived from Zea mays GSH-S sequence Q8W4W2

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 100 µl
Reconstitution For reconstitution add 100 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications
Immunolocalization (IL), Western Blot (WB)
Related products
AS06 181 | anti-GR | glutathione reductase

Additional information

Total IgG concentration is 3.7 µg/µl

application information
Recommended dilution

1:2 000 with standard ECL (WB)

Expected | apparent MW

45 kDa

Confirmed reactivity

Nicotiana tabacum, Solanum lycopersicum, Zea mays

Predicted reactivity

dicots including: Arabidopsis thaliana, Pisum sativum, monocots including: Hordeum vulgare, Oryza sativa

Not reactive in

No confirmed exceptions from predicted reactivity known in the moment

Additional information

Immunolocalization has been done for Arabidopsis thaliana and Nicotiana tabacum

Selected references Jayawardena et al. (2016). Elevated CO2 plus chronic warming reduces nitrogen uptake and levels or activities of nitrogen -uptake and -assimilatory proteins in tomato roots. Physiol Plant. 2016 Nov 28. doi: 10.1111/ppl.12532. [Epub ahead of print]
Baojian et al. (2014). Maize (Zea mays L.) seedling leaf nuclear proteome and differentially expressed proteins between a hybrid and its parental lines. Proteomics, DOI: 10.1002/pmic.201300147.
Gomez et al. (2004) Intercellular distribution of glutathione synthesis in maize leaves and its response to short term chilling. Plant Physiol. 134: 1662-1671.


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