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GS | Glutathione synthase, GSH-S

345 €

AS06 183  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: Nicotiana tabacum, Solanum lycopersicum, Zea mays

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AS06 183

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product information
Background

Glutathione synthetase (GSH-S) catalyses the last step of glutathione (GSH) synthesis. GSH is synthesized in two ATP-dependent steps: in the first, gamma-glutamylcysteine (gamma-EC) is synthesized from L-glutamate and cysteine, catalyzed by gamma-glutamylcysteine synthetase (g-ECS); in the second step, catalysed by GSH-S, glycine is added to the C-terminal end of gamma-EC. GSH is the predominant non-protein thiol in all nearly where it acts as an antioxidant in stress responses and as a mobile pool of reduced sulfur. It is also important in the regulation of plant growth and development

Immunogen

KLH-conjugated synthetic peptide derived from Zea mays GSH-S sequence Q8W4W2

Host Rabbit
Clonality Polyclonal
Clone
Purity Total IgG
Format Lyophilized in PBS pH 7.4
Quantity 100 µl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Immunolocalization (IL), Western blot (WB)
Related products
AS06 181 | anti-GR | glutathione reductase

Additional information

Total IgG concentration is 3.7 µg/µl

application information
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW

45 kDa

Confirmed reactivity Nicotiana tabacum, Solanum lycopersicum, Zea mays
Predicted reactivity Aegilops tauschii, Arabidopsis thaliana, Arundo donax, Asparagus officinalis, Brachypodium distachyon, Capsicum annuum, Cicer arietinum, Citrus clementina, Corchorus olitorius, Cucumis sativus, Daucus carota subsp. sativus, Dimocarpus longan, Eucalyptus grandis, Gossypium hirsutum , Hevea brasiliensis, Hordeum vulgare, Jatropha curcas, Lotus japonicus, Lupinus angustifolius, Lycium chinense, Musa acuminata, Nelumbo nucifera, Nicotiana tabacum, Nicotiana sylvestris, Oryza sativa, Pisum sativum, Setaria italica, Sorghum bicolor, Spinacia oleracea, Triticum aestivum, Zostera marina , Vitis vinifera
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information

Immunolocalization has been done for Arabidopsis thaliana and Nicotiana tabacum

Selected references Jayawardena et al. (2016). Elevated CO2 plus chronic warming reduces nitrogen uptake and levels or activities of nitrogen -uptake and -assimilatory proteins in tomato roots. Physiol Plant. 2016 Nov 28. doi: 10.1111/ppl.12532. [Epub ahead of print]
Baojian et al. (2014). Maize (Zea mays L.) seedling leaf nuclear proteome and differentially expressed proteins between a hybrid and its parental lines. Proteomics, DOI: 10.1002/pmic.201300147.
Gomez et al. (2004) Intercellular distribution of glutathione synthesis in maize leaves and its response to short term chilling. Plant Physiol. 134: 1662-1671.


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