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V-ATPase, D | Vacuolar H+-ATPase subunit D

454 €

AS09 497 | clonality: polyclonal | host: rabbit | reactivity: Arabidopsis thaliana

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AS09 497

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product information
Background

V-ATPase is an enzyme responsible for acidification of intracellular compartments. Subunit D is the peripheral subunit of V1 complex. Alternative name: Vacuolar proton pump subunit D, vacuolar H(+)-ATPase subunit D

Immunogen

KLH-conjugated synthetic peptide conserved in Arabidopsis thaliana V-ATPase subunit D Q9XGM1, At3g58730

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format
Quantity 100 ĩl
Reconstitution
Storage

store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), western blot (WB)
Related products

collection of antibodies to vacuolar proteins

AS09 466 | anti-V-ATPase subunit a | vacuolar H+ ATPase, subunit a

AS09 467 | anti-V-ATPase subunit A | vacuolar H+-ATPase

AS09 503 | anti-V-ATPase, B | vacuolar ATP synthase subunit beta

AS09 468 | anti-V-ATPase subunit c | vacuolar H+-ATPase, subunit c (16 kDa)

ASS09 497 | anti- V-ATPase subunit D |V-type proton ATPase subunit D

AS07 213 | anti-V-ATPase subunit E of tonoplast H+ATPase

AS09 499 | anti-V-ATPase subunit H |V-type proton ATPase subunit H

Plant protein extraction buffer

Secondary antibodies

Additional information

0.1 % sodium azide is added as preservative. For antibody re-suspending information check the tube lable.

Antibodies will detect target protein in a few µg of a crude preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, one µg load per well should be sufficient.

Protocol of isolation of vacuolar membranes can be found here.

application information
Recommended dilution

1: 8000 (ELISA), 1: 2000 with standard ECL (WB)

Expected | apparent MW

29 | 29 kDa

Confirmed reactivity

Arabidopsis thaliana

Predicted reactivity

dicots including: Sorghum bicolor, monocots including: Zea mays

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

Diluted antibody solution can be used 2 to 3 times within one month if it contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.

Manufactured by Operon Biotechnologies.

Selected references

to be added when avaialble



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