FOX1 | plasma membrane ferroxidase FLP1
AS06 120 | clonality: polyclonal | host: rabbit | reactivity: Chlamydomonas reinhardtii
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|Recommended dilution||1 : 1000-1 : 5000 (WB)|
|Expected | apparent MW||
122 | 120 kDa
|Confirmed reactivity||Chlamydomonas reinhardtii|
|Predicted reactivity||Chlamydomonas reinhardtii|
|Not reactive in||No confirmed exceptions from predicted reactivity are currently known.|
This antibody has been used on Chlamydomonas cells expressing endogenous Fox1 and on HEK293 cells transiently expressing Fox1. Cells were resuspended in PBS plated on poly-lysine coated coverslips, fixed in 3.7% formaldehyde. Blocked in 3% BSA in PBS. 1:500 dilution of anti-FOX1 in 3% BSA. Secondary antibody was anti-rabbit Alexa fluor 488 1:300.
|Selected references||Kropat et al. (2015). Copper economy in Chlamydomonas: Prioritized allocation and reallocation of copper to respiration vs. photosynthesis. Proc Natl Acad Sci U S A. 2015 Feb 2. pii: 201422492.
LaFontaine et al. (2002) Copper-Dependent Iron Assimilation Pathway in the Model Photosynthetic Eukaryote Chlamydomonas reinhardtii. Eukaryotic Cell 1(5):736-757
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