PsbD | D2 protein of PSII positive control/quantitation standard
AS09 146S | Recombinant protein standard for quantitation and positive control
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Standard curve: 3 loads are recommended (0.5, 2 and 4μl).
For most applications a sample load of 0.2 μg of chlorophyll will give a PsbD signal in this range.
Positive control: a 2 μl load per well is optimal for most chemiluminescent detection systems.
This standard is stabilized and ready and does not require heating before loading on the gel.
Please note that this product contains 10% glycerol and might appear as liquid but is provided lyophilized. Allow the product several minutes to solubilize after adding water. Mix thoroughly but gently Take extra care to mix thoroughly before each use, as the proteins tend to settle with the more dense layer after freezing.
|Expected | apparent MW||
In most gel systems PsbD migrates around 28-30 kDa
|Not reactive in|
Concentration: after adding 225 µl of milliQ water final concentration of the standard is 0.25 pmoles/ul
|Selected references||Li et al. (2016). A Hard Day's Night: Diatoms Continue Recycling Photosystem II in the Dark. Front. Mar. Sci., 08 November 2016
Li et al. (2014). The nitrogen costs of photosynthesis in a diatom under current and future pCO2. New Phytol. 2014 Sep 25. doi: 10.1111/nph.13037.
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