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PsaA | PSI-A core protein of photosystem I

265 €
Buy 2 items of this product for 198 €/each
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AS06 172  |  Clonality: Polyclonal  |  Host: Rabbit  |  Reactivity: A. thaliana, C. quitensis Kunt Bartl, C. pumilum C. reinhardtii, F. vesiculosus, H.vulgare, M. polymorpha, N. tabacum, O. sativa, P. abies, P. sativum, P. strobus, P. vulgaris, S. oleracea, C.reinhardtii, Synechococcus  PCC 7942,  Synechocystis PCC 6803, Scenedesmus obliquus, microalgae N. gaditana

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AS06 172

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product information
Background

PsaA is a core protein of photosystem I. In plants and cyanobacteria, the primary step in oxygenic photosynthesis, the light induced charge separation, is driven bytwo large membrane intrinsic protein complexes, the photosystems I and II.  Synonym: Photosystem I P700 chlorophyll a apoprotein A1.      

Immunogen

N-terminal part of recombinant PsaA protein from Chlamydomonas reinhardtii P12154

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 50 µl
Reconstitution For reconstitution add 50 µl of sterile water.
Storage

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications Immunogold (IG), Western Blot (WB), Bue Native PAGE (BN-PAGE)
Related products

Collection of antibodies to PSI proteins

recommended secondary antibody

Plant and algal protein extraction buffer

Secondary antibodies

Additional information

PsaA is a hydrophobic protein and we recommend to use PVDF membrane for transfer to assure best results.

This product can be sold containing ProClin if requested

application information
Recommended dilution

1: 20 (IG), 1: 1000 - 1: 5000 (WB)

Expected | apparent MW

82 | 55-60 kDa

Confirmed reactivity Arabidopsis thaliana, Bryopsis corticulans, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Craterostigma pumilum, Cytisus cantabricus (Wilk.) Rchb. F., Drosera capensis, Fucus vesiculosus, Haematococcus pluvialis, Hieracium pilosella L., Hordeum vulgare, Lasallia hispanica, Nicotiana tabacum, Oryza sativa, Pisum sativum, Marchantia polymorpha (liverwort), Phaseolus vulgaris, Physcomitrella patens, Picea abies, Pinus strobus, Sinapsis alba, Spinacia oleracea, Chlamydomonas reinhardtii, Synechococcus PCC 7942, Synechocystis PCC 6803, Syntrichia muralis (Hedw.) Raab, Scenedesmus obliquus, microalgae: Nannochloropsis gaditana
Predicted reactivity

dicots including: Lycopersicum esculentum, Panax ginseng, monocots including: Triticum aestivum 

trees: Picea spinulosa, Pinus thunbergii, Populus alba, Citrus x limon

Bigelowiella natans, algae, cyanobacteria

Not reactive in

Chromera velia

Additional information

Immunogold localization has been done in leaf material of  Arabidopsis thaliana.

This product can be sold containing ProClin if requested.

Selected references Fu et al. (2017). Redesigning the QA binding site of Photosystem II allows reduction of exogenous quinones. Nat Commun. 2017 May 3;8:15274. doi: 10.1038/ncomms15274. (Chlamydomonas reinhardtii)
Sakuraba et al. (2017). Rice Phytochrome-Interacting Factor-Like1 (OsPIL1) is involved in the promotion of chlorophyll biosynthesis through feed-forward regulatory loops. Journal of Experimental Botany doi:10.1093/jxb/erx231.
Gandini et al. (2017). The transporter SynPAM71 is located in the plasma membrane and thylakoids, and mediates manganese tolerance in Synechocystis PCC6803. New Phytol. 2017 Mar 20. doi: 10.1111/nph.14526. (BN-PAGE)
Míguez et al. (2017). Diversity of winter photoinhibitory responses: A case study in co-occurring lichens, mosses, herbs and woody plants from subalpine environments. Physiol Plant. 2017 Feb 14. doi: 10.1111/ppl.12551.
Mazur et al. (2016). Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity. BMC Plant Biol. 2016 Sep 2;16(1):191. doi: 10.1186/s12870-016-0883-4.
Yoshida et al. (2016). Hisabori T1.Two distinct redox cascades cooperatively regulate chloroplast functions and sustain plant viability. Proc Natl Acad Sci U S A. 2016 Jul 5;113(27):E3967-76. doi: 10.1073/pnas.1604101113. Epub 2016 Jun 22.
Gerotto et al. (2016). Flavodiiron proteins act as safety valve for electrons in Physcomitrella patens. PNAS DOI 10.1073.
Pavlovič et al. (2016). A carnivorous sundew plant prefers protein over chitin as a source of nitrogen from its traps. Plant Physiol Biochem. 2016 Mar 5;104:11-16. doi: 10.1016/j.plaphy.2016.03.008
Pavlovič et al. (2016). Light-induced gradual activation of photosystem II in dark-grown Norway spruce seedlings. Biochim Biophys Acta. 2016 Feb 18. pii: S0005-2728(16)30028-7. doi: 10.1016/j.bbabio.2016.02.009.
Pinnola et al. (2015). Light-Harvesting Complex Stress-Related Proteins Catalyze Excess Energy Dissipation in Both Photosystems of Physcomitrella patens. Plant Cell. 2015 Nov;27(11):3213-27. doi: 10.1105/tpc.15.00443. Epub 2015 Oct 27.
Zhang et al. (2015). Subcellular Localization of Carotenoid Biosynthesis in Synechocystis sp. PCC 6803. PLoS One. 2015 Jun 17;10(6):e0130904. doi: 10.1371/journal.pone.0130904.
Gerotto et al. (2015). In Vivo Identification of Photosystem II Light Harvesting Complexes Interacting with PHOTOSYSTEM II SUBUNIT S. Plant Physiol. 2015 Aug;168(4):1747-61. doi: 10.1104/pp.15.00361. Epub 2015 Jun 11.
Liu and Last (2015). A land plant-specific thylakoid membrane protein contributes to photosystem II maintenance in Arabidopsis thaliana. Plant J. 2015 Jun;82(5):731-43. doi: 10.1111/tpj.12845. Epub 2015 Apr 29.
Dahal et al. (2015). Improved photosynthetic performance during severe drought in Nicotiana tabacum overexpressing a nonenergy conserving respiratory electron sink. New Phytol. 2015 May 29. doi: 10.1111/nph.13479.
Knuesting et al. (2015). Arabidopsis glutaredoxin S17 and its partner, the nuclear factor Y subunit C11/negative cofactor 2α, contribute to maintenance of the shoot apical meristem under long-day photoperiod. Plant Physiol. 2015 Apr;167(4):1643-58. doi: 10.1104/pp.15.00049. Epub 2015 Feb 19.
Charuvi et al. (2015). Photoprotection Conferred by Changes in Photosynthetic Protein Levels and Organization during Dehydration of a Homoiochlorophyllous Resurrection Plant. Plant Physiol. 2015 Apr;167(4):1554-65. doi: 10.1104/pp.114.255794.
Wang et al. (2014). Cellular Capacities for High-Light Acclimation and Changing Lipid Profiles across Life Cycle Stages of the Green Alga Haematococcus pluvialis. PLoS One. 2014 Sep 15;9(9):e106679. doi: 10.1371/journal.pone.0106679. eCollection 2014.
Lin et al. (2014). Analysis of an Arabidopsis Heat-sensitive Mutant Reveals that Chlorophyll Synthase is Involved in Reutilization of Chlorophyllide during Chlorophyll Turnover. Plant J. 2014 Jul 8. doi: 10.1111/tpj.12611.

Application example
western blot using anti-PsaA antibodies
2 µg of total protein from (1) Arabidopsis thaliana leaf, (2) Hordeum vulgare leaf, (3) Chlamydomonas reinhardtii total cell, (4) Synechococcus sp. 7942 total cell all extracted with Protein Extration Buffer, PEB (AS08 300), were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, frecommended secondary antibody AS09 602) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 10 seconds.


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