Animal cell antibodies / Fish proteins

product information

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery.

KLH-conjugated synthetic peptide chosen from the C-terminal of salmonid hsp70. The target peptide is a sequence specific to salmonid hsp70 B5X4Z3.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB), immunoprecipiation (IP), immunohistochemistry (IHC)

collection of antibodies to fish related targets

immunohistochemistry experiments have been done on salmon tissue treated with hydrated autoclaving of formalin fixed sections (unpublished results)

application information

1:15 000 with standard ECL (WB), 5 µg of antibodies in reaction mixture (IP), 1: 100 (IHC)

70 kDa

rainbow trout (Oncorhynchus mykiss)

atlantic salmon (Salmo salar), brook trout (Salvelinus fontinalis)

no confirmed exceptions from predicted reactivity known in the moment

not available at the moment

Curie et al. (2008). β‐Adrenergic Stimulation Enhances the Heat‐Shock Response in Fish. Physiol & Bioch. Zoology 4:414-425.