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HSP70 | salmonid heat shock protein 70

345 €

AS05 061 |  clonality: polyclonal  |  host: rabbit  |  reactivity: Salmo sp. (salmon), Salvelinus fontinalis (brook trout)

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Item No:
AS05 061

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product information
Background

Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery.

Immunogen

KLH-conjugated synthetic peptide chosen from the C-terminal of salmonid hsp70. The target peptide is a sequence specific to salmonid hsp70 B5X4Z3.

Host Rabbit
Clonality Polyclonal
Clone
Purity Serum
Format Lyophilized
Quantity 100 ĩl
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

collection of antibodies to fish related targets

Secondary antibodies

Additional information

The antibody is very specific for salmonid inducible form - hsp70. It does not cross-react with hsc70. It does not detect hsp70 from other species.

application information
Recommended dilution

1:5 000 with standard ECL (WB)

Expected | apparent MW

70 kDa

Confirmed reactivity rainbow trout (Oncorhynchus mykiss), brook trout gills (Salvelinus fontinalis)
Predicted reactivity

atlantic salmon (Salmo salar), 

Not reactive in

no confirmed exceptions from predicted reactivity known in the moment

Additional information

not available at the moment

Selected references Ricketts et al. (2015). The Effects of Acute Waterborne Exposure to Sublethal Concentrations of Molybdenum on the Stress Response in Rainbow Trout, Oncorhynchus mykiss. PLoS One. 2015 Jan 28;10(1):e0115334. doi: 10.1371/journal.pone.0115334. eCollection 2015.
Templeman et al. (2014). Linking physiological and cellular responses to thermal stress: β-adrenergic blockade reduces the heat shock response in fish. J Comp Physiol B, April 2014.


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