GLN1 GLN2 | GS1 GS2 glutamine synthetase global antibody

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AS08 295 | clonality: polyclonal | host: rabbit | reactivity: [global antibody] | A. thaliana, E. tef, M. truncatula, S. oleracea, P.patens, Z. mays red alga Gracilaria gracilis


13 st
Item No:
AS08 295

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product information

Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN1 is highly abundant in the vascular elements of roots nodules, flowers and fruits, functioning in the assimilation of ammonium and the biosynthesis of glutamine for nitrogen transport. GLN2 is encoded by a single gene and is highly abundant in leaf mesophyll chloroplasts. Here GLN functions in the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts


KLH-conjugated synthetic peptide derived from a wide range of available sequences including all isoforms of Arabidopsis thaliana GLN1-1,1-2,1-3 and 1-4, (At5g37600, At1g66200, At3g17820, At5g16570)

Host Rabbit
Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl of sterile water.

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications western blot (WB)
Related products

AS08 295PRE | GLN1 GLN2 | GS1 glutamine synthetase, pre-immune serum

AS08 296 GLN | GS2, chloroplastic form of glutamine synthetase

collection of antibodies to proteins involved in nitrogen metabolism

plant and algal protein extraction buffer

secondary antibodies

Additional information

The antibody will recognize both, cytoplasmic and chloroplastic forms of the GS enzyme.

application information
Recommended dilution

1:10 000 (WB)

Expected | apparent MW

39-40 kDa (GLN1,cytoplasmic form) , 44-45 kDa (GLN2, chloroplastic form)

Confirmed reactivity

Arabidopsis thaliana, Eragrostis tef, Medicago truncatula, Spinacia oleracea, Zea mays, moss: Physcomitrella patens, red alga Gracilaria gracilis

Predicted reactivity

Brachypodium distachyon, Brassica napus, Camellia sinensis, Citrus clementina, Daphnia magna, Datisca glomerata, Emiliania huxleyi, Eucalyptus grandis, Gazania splendens, Genlisea aurea, Glycine max, Helianthus annuus, Hordeum vulgare, Oryza sativa, Panax quinquefolius, Phaseolus angularis, Phytophthora cinnamomi, Populus trichocarpa, Saccharum officinarum, Securigera parviflora, Solanum lycopersicum, Solanum tuberosum, Stevia rebaudiana, Theobroma cacao, Triticum aestivum, Zea mays, Vitis labrusca

GLN1 dicots including: Brassica napus, Phaseolus vulgaris, monocots including: Hordeum vulgare, Oryza sativa, trees: Pinus sylvestris, Populus sp., Zosteria marina

GLN2 dicots including: Brassica napus, Glycine max, Phaseolus vulgaris, monocots including: Triticum aestivum, Oryza sativa

GLN3: Zea mays,

GLN1 in algae: Chlamydomonas reinhardii

Not reactive in

no confirmed exceptions from predicted reactivity known presently

Additional information

to be added when available

Selected references Silva et al. (2015). Possible role of glutamine synthetase of the prokaryotic type (GSI-like) in nitrogen signaling in Medicago truncatula. Volume 240, November 2015, Pages 98–108.
Podgórska et al. (2013). Long-term ammonium nutrition of Arabidopsis increases the extrachloroplastic NAD(P)H/NAD(P)+ ratio and mitochondrial reactive oxygen species level in leaves but does not impair photosynthetic capacity. Plant Cell Environ. April 10.

Brouwer et al. (2011) The Impact of Light Intensity on Shade-Induced Leaf Senescence. Plant Cell Environ. Dec. 15 (ahead of print).
Lang et al. (2011).Simultaneous isolation of pure and intact chloroplasts and mitochondria from moss as the basis for sub-cellular proteomics. Plant Cell Rep. Feb;30(2):205-15. (reactivity confirmed for Physcomitrella patens).

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Accessories: GLN1 GLN2 | GS1 GS2 glutamine synthetase global antibody

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