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AS12 2220 | clonality: polyclonal | host: rabbit | reactivity: Human influenza hemagglutinin (HA) tagged proteins


109 st
Item No:
AS12 2220

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product information
Background HA-tag is derived from a human influenza hemagglutinin HA-molecule corresponding to amino acids 96-106 and is used as a general epitope tag in expression vectors.

KLH-conjugated synthetic peptide: YPYDVPDYA

Host Rabbit
Clonality Polyclonal
Purity Affinity purified serum in PBS, pH 7.4
Format Lyophilized
Quantity 100 ĩg
Reconstitution For reconstitution add 100 ĩl of sterile water.
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications Western blot (WB)
Related products

AS11 1771 | anti-His-tag | 6xHis mouse antibody

AS09 601 | anti-c-Myc chicken antibody

Plant and algal protein extraction buffer

Secondary antibodies

Additional information
application information
Recommended dilution 1 : 5000 (WB)
Expected | apparent MW

depends on the MW of the protein which is HA-tagged

Confirmed reactivity HA-tag
Predicted reactivity
Not reactive in No confirmed exceptions from predicted reactivity are currently known.
Additional information
Selected references

to be added when available, antibody released in Febraury 2013.

application example


western blot using anti-HA tag antibody on plant recombinant protein

 Total protein was extracted from WT and a transgenic line of Arabidopsis thaliana expressing a HA-tagged chloroplast protein.  The extraction buffer contained 0.2M Tris-HCl pH 6.8, 10% Glycerol, 8% SDS, 20% bME.  Proteins were separated on 11% SDS-PAGE, and blotted onto a nitrocellulose membrane. The blot was blocked with 5% non-fat dry milk in 1x TBS-T buffer for 1h at room temperature (RT) with agitation. The blot was incubated with the primary antibody at a dilution of 1: 4 000 for 1h at RT with agitation. The antibody solution was decanted, the blot was rinsed briefly, and washed 3 times (5, 10 and 15 min) in TBS-T at RT with agitation. The blot was then incubated in a secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated; Agrisera, AS09 602, 1:10 000 dilution) for 1h at RT with agitation. The blot was washed as above and developed for 5 min with ECL according to the manufacturer instructions. Signal was captured using the GE LAS500 instrument, with exposure time of 30 seconds.

Courtesy of Dr. Zach Adam, The Hebrew University of Jerusalem, Israel

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