Plant/Algal cell antibodies / Populus sp.

product information

Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN2 is encoded by a single gene and is highly abundant in mesophyll cells of leaves for the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts.

KLH-conjugated synthetic peptide which is a part of part of the glutamine synthetase/guanido kinase superfamily catalytic region

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

western blot (WB)

AS08 295 GLN1 GLN2 | GS1 glutamine synthetase global antibody

to be added when available

application information

1:5000 on 0.5-5 ug protein/lane with ECL Advance detection (WB)

47 | 44-45 kDa

Arabidopsis thaliana, Pisum sativum, Spinacia oleracea

dicots including: Brassica napus, Glycine max,  Phaseolus vulgaris, monoctos including: Hordeum vulgare, Oryza sativa,Triticum, aestivum, Zea mays, trees: Pinus sylvestris, Populus sp.,  moss: Physcomitrella patens,

no confirmed exceptions from predicted reactivity known in the moment

to be added when available

to be added when available

application example

0.5 µg of protein from Arabidopsis thaliana total leaf fraction (1), 5 µg of protein from Spinacia oleracea chlorplast enriched fraction (2), molecular weight markers (MagicMark^TM,Invitrogen) (M), the same samples as in 1 and 2 but after peptide neutralisation assay, e.g.  incubation of the antibody with 100 mM excess of peptide used to elicit andt-GLN2 antibody (4,5), extracted with PEB (AS08 300), were separated on  4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF (Millipore). Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-GLN2 antibody (AS08 296, 1:5 000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T. All steps were performed at RT with agitation. Blots were developed for 5 min with ECL Advance detection reagent according the manufacturers instructions (GE Healthcare). Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).