GLN2 | GS2, chloroplastic form of glutamine synthetase

320 €

AS08 296 | clonality: polyclonal | host: rabbit | reactivity: A. thaliana, P. sativum, S. oleracea

product information

background

Glutamine synthetase (GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN2 is encoded by a single gene and is highly abundant in mesophyll cells of leaves for the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts. GLN2 is a target for thioredoxin.

immunogen

KLH-conjugated synthetic peptide which is a part of part of the glutamine synthetase/guanido kinase superfamily catalytic region

antibody format

rabbit

polyclonal

serum

lyophilized

quantity

400 µl

for reconstitution add 400 µl of sterile water.

storage

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

tested applications

western blot (WB)

related products

AS08 295 GLN1 GLN2 | GS1 glutamine synthetase global antibody

additional information

to be added when available

application information
recommended dilution		1:5000 on 0.5-5 ug protein/lane with ECL Advance detection (WB)
expected \| apparent MW		47 \| 44-45 kDa
confirmed reactivity		Arabidopsis thaliana, Pisum sativum, Spinacia oleracea
predicted reactivity		dicots including: Brassica napus, Glycine max, Phaseolus vulgaris, monoctos including: Hordeum vulgare, Oryza sativa,Triticum, aestivum,Zea mays,trees: Pinus sylvestris, Populus sp., moss: Physcomitrella patens,
not reactive in		diatoms
additional information		to be added when available
selected references		Lee et al. (2013). Stromal protein degradation is incomplete in Arabidopsis thaliana autophagy mutants undergoing natural senescence. BMC Res Notes, Jan 17.

application example

0.5 µg of protein from Arabidopsis thaliana total leaf fraction (1), 5 µg of protein fromSpinacia oleracea chlorplast enriched fraction (2), molecular weight markers (MagicMark^TM,Invitrogen) (M), the same samples as in 1 and 2 but after peptide neutralisation assay, e.g. incubation of the antibody with 100 mM excess of peptide used to elicit andt-GLN2 antibody (4,5), extracted with PEB (AS08 300), were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF (Millipore). Filters were blocked 1h with 2% low-fat milk powder in TBS-T (0.1% TWEEN 20) and probed with anti-GLN2 antibody (AS08 296, 1:5 000, 1h) and secondary anti-rabbit (1:20000, 1 h) antibody (HRP conjugated, Abcam) in TBS-T containing 2% low fat milk powder. Antibody incubations were followed by washings in TBS-T. All steps were performed at RT with agitation. Blots were developed for 5 min with ECL Advance detection reagent according the manufacturers instructions (GE Healthcare). Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad).

Western blot detection using anti-GLN2 antibodies

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