Agrisera blog

Secondary antibodies conjugated to HRP (Horseradish peroxidase) and ALP (Alkaline phosphatase) enzymes are most frequently used to visualize target proteins in Western blot, ELISA and immunolocalization. When using secondary antibodies conjugated to HRP, the detection is performed using chemiluminescence (recorded by a CCD camera or another imager), which gives high sensitivity and good resolution in both Western blot and chemiluminescent ELISA. When using antibodies conjugated to ALP, visualization of the antibody binding to the target is achieved using chromogenic reagents, and no CCD camera is necessary.  

Agrisera's secondary antibody collection is composed of over 220 antibodies; both highly purified and available in a basic format, suitable for Western blot, as well as adsorbed to serum from a wide range of animal species, suitable for immunolocalization and ELISA. Based on the analysis of antibody performance in available publications, conducted by CiteAB, it became very clear that Agrisera secondary antibodies are performing above market average. This means that Agrisera secondary antibodies conjugated to ALP or HRP can be used at high dilutions and are price-worthy reagents, and will provide you with a consistent performance over many experiments.

Broad species reactivity

• Broad species reactivity, including both di- and monocols and microalgae
• Can be used in dilutions of >1: 1000 in Western blot
• Extensive publication record
• Promptly and environmentally-friendly delivery

Agrisera will participate in the following meetings in 2023:

3^rd INUPRAG Symposium on Integrative Plant Biology, 21^st-22^nd of February, Umeå Plant Science Centre, Umeå, Sweden
The 33^rd International Conference on Arabidopsis Research, 5^th-9^th of June, Chiba, Japan
Nordic Photosynthesis Congress and Nordic Algae Symposium, 19^th-21^st of June, Umeå, Sweden
Plant Biology Europe, 3^rd-6^th of July, Marseille, France
Plant Biology 2023 (ASPB), 5^th-8^th of August, Savannah, Georgia, USA
11^th Conference of Polish Society of Experimental Plant Biology, 19^th-22^nd of September, Poznań, Poland
KBC Days 2023, November, Umeå University, Sweden 

Agrisera supports the following meetings in 2023:

Umeå Center for Microbial Research Day (UCMR Day 2023), 19^th of January, Umeå University, Sweden
36^th Molecular Biology of Plants (MBP2023), 6^th-9^th of February, Bonn, Germany
20^th Scientific Conference of the Phycology Section, 12^th-14^th of March, Jena, Germany
19th International Workshop on Plant Membrane Biology, 27^th-31^st of March, Taipei, Taiwan
ATSPB 2023: Plants in the context of food security, biodiversity and climate change, 13^th-15^th of April, Tirol, Austria
32^nd Western Photosynthesis Conference, 28^th-30^th of April, Bodega Bay, California, USA
5^th Conference of the International Plant Proteomics Organization, 22^nd-25^th of May, Thesaloniki, Greece
IPG 2023 Symposium - Redox Regulation of Plant Stress and Development, 23^th - 26^th of May, University of Missouri, USA
6^th UNIGE PostDoc Day, 8^th of June at the University of Geneva, Switzerland
Chlamy2023, The 20th International Conference on the Cell and Molecular Biology of Chlamydomonas, 4^th-9^th of June, Princeton University, New Jersey, USA
Plant Biology 2023, XXV Meeting of the Spanish Society of Plant Biology, 9^th-12^th of July, Braga, Portugal
4^th International Brassinosteroid Conference, 16^th-18^th of August, Ghent, Belgium
Plants and People, 6t^h-7^th of September, Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany
Research Day, 20^th of September, Oslo, Norway
11^th Conference of Polish Society of Experimental Plant Biology, 19^th-22^nd of September, Poznań, Poland
2^nd International Conference on Plant Systems Biology and Biotechnology (ICPSBB), 25^th-30^th of September, Plovdiv, Bulgaria
12^th SPPS PhD conference PhD 101: Everything is hard before it gets easy, 27^th-29^th of September, Lund, Sweden
4^th Annual Congress of Young Researchers (ACYR), 9^th-10^th of October, CRAG auditorium, Spain
49th Midwest/Southeast Photosynthesis Conference, , 27^th-29^th of October, Turkey Run State Park, USA
The Canadian Society of Plant Biologists (CSPB), Eastern Regional Meeting, 1^st-2^nd of December, Concordia University, Montreal, Canada.