Agrisera blog

Agrisera antibodies in immunolocalization studies overview

The conference Straub Days 2023, took place between 25th and 26th of May at the Biological Research Centre, Szeged, Hungary. During the conference, the poster "Plant cell immunolocalization and the use of advanced imaging techniques at HCEMM Advanced Core Facility" was presented by Dr. Ferhan Ayaydin and Dr. Divya Teja Dondapati from the Functional Cell Biology and Immunology-Advanced Core Facility, HCEMM, Szeged; and Institute of Plant Biology, BRC, Szeged.

The poster showed various methods of material preparaton, and included immunolocalization results obtained with Agrisera antibodies to:

Actin (AS22 4615)
Clathrin heavy chain 1,2 (AS10 690)
Histone 3 (AS10 710A), and
GFP (AS21 4443)

These were combined with matching Agrisera secondary antibodies:

Donkey anti-mouse IgG (H&L), DyLight® 488-conjugated secondary antibodies (AS10 1261), and
Donkey anti-Rabbit IgG (H&L), DyLight® 550-conjugated secondary antibody (AS12 1962)

The plant material used were: Oryza sativa L. ssp. japonica cv. 'Unggi 9', Zea mays hybrid variety, Arabidopsis thaliana expressing GFP, and Oryza sativa bpt-jeelakarra variety.

Sample preparation methods followed for the immunolocalization studies on dicot and monocots were: suspension culture, root squash, whole root mount and cryosectioning.

Immunolocalization with Agrisera antibodies shown on a poster during Straub Days in Szeged, Hungary

Seen in the image, left to right: Dr. Ferhan Ayaydin and Dr. Divya Teja Dondapati.

Fast and easy protein extraction using Agrisera Protein Extraction Buffer (PEB)

Protein Extraction Buffer (PEB) is an extraction buffer for the disruption and solubilization of total protein from plant tissues and algal cells. The buffer contains the anionic detergent LDS, which does not form crystals while stored at 4-10°C, and is quickly ready to use.

Expected yields will be 1.5-6 µg/µl total protein (recovered from standard procedure), depending on the starting material, e.g. its biological stage and homogenization method used (bead beater vs. sonication). For processing of multiple samples, simultaneous extraction using for example a bead beater is recommended, for consistency of extraction.

Provided as 5 x 2 ml (4x stock), Agrisera's PEB buffer allows up to 75 isolations of plant material (using 500 μl 1x PEB for 100 mg fresh weight), or 190 isolations of algal material (using 200 μl 1x PEB for cell amounts corresponding to 4-10 μg total chlorophyll).

To product page:
PEB (4x) | protein extraction buffer

Chosen references:
• Altuntas et al. (2020). Proline-stimulated signaling primarily targets the chlorophyll degradation pathway and photosynthesis associated processes to cope with short-term water deficit in maize. Photosynth Res. 2020 Apr;144(1):35-48. doi: 10.1007/s11120-020-00727-w.
• Pérez-López et al. (2020). Transcriptome Analysis Identifies Plasmodiophora brassicae Secondary Infection Effector Candidates. J Eukaryot Microbiol. 2020 Jan 11. doi: 10.1111/jeu.12784.
• Morin et al. (2019). Morin et al. (2019). Response of the sea-ice diatom Fragilariopsis cylindrus to simulated polar night darkness and return to light. Limnology and Oceanography. 9999, 2019, 1Ã¢??20. (sea-ice diatom)
• Bausch, A.R., Juhl, A.R., Donaher, N.A. et al. Mar Biol (2019) 166: 80.

Agrisera Western blot training for Bicom, Agrisera distributor in South Africa

On the 26th of May, Dr. Joanna Porankiewicz Asplund, Technical Support Manager at Agrisera, conducted a Western blot training for the sales force of Agrisera's distributor in South Africa, Bicom Biotech.

The aim was to show which are the most crucial steps of Western blot, and how to help customers using this widely employed protein analysis technique.

Good luck with helping your customers succeed with their Western blots!

Extensive collection of antibodies to Arabidopsis thaliana proteins involved in a stress response

As climate change becomes more noticeable, and affect all life on Earth, research on how plants respond to environmental stress intensifies in many laboratories worldwide. Agrisera antibodies, to key proteins involved in plant stress response, help solve many pressing questions of how plants tackle changes to, and threats in, their environment. New antibodies are added continuously to our collection, which includes antibodies to proteins involved in:

Cold stress | Drought stress | Heat shock | Heavy metals | Hypoxia | Oxidative stress | Pathogen attack | Salt stress | UV radiation | Wounding

Agrisera environmental stress antibodies

Agrisera Western blot workshop for Wageningen University & Research Laboratory of Biochemistry

On the 15th of May, Dr. Joanna Porankiewicz-Asplund, Agrisera's Technical Support Manager, conducted an online workshop for researchers from the Wageningen University & Research Laboratory of Biochemistry, The Netherlands. The title of the workshop was "Western blot - a technique possible to optimize".

The workshop was almost two hours long, including a 10 minutes break for the Agrisera Antibody and Western blot Quizzes and coffee. Questions submitted by attendees were answered, and many pitfalls of the Western blot technique were discussed, with specific experimental examples.

Participants also had a chance to win Agrisera high titer secondary antibodies and 2 ECL reagents, in two quizzes, consisting of questions about antibodies and the Western blot technique.

Each participant of the workshop received a pdf of the presentation with links to additional information, as well as a pdf of Agrisera's Western blot guide. The recording of the workshop is available on demand.

If you are interested in this type of online workshop to be held at your institution, please contact us!

Western blot technique possible to optmize Wageningen University

Feedback from one of the participants: "Thanks! It was a very nice and informative workshop :)"

Free epitope tag antibodies

Agrisera offers free-of-charge epitope tag antibodies in return for results. Below is a list of epitope tag antibodies available for testing.

Beside 50 µg of antigen-purified antibodies, we will also provide you with:

High-titer, matching secondary antibodies, to be used at 1: 25 000, 1h/RT incubation
Agrisera ECL reagents of mid-picogram or extreme low femtogram detection range
Suggested protocol

For Western blot, ChIP and immunolocalization.

Product number	Product name	Uniprot ID	Research area
AS21 4612	BAR \| Phosphinothricin N-acetyltransferase (170-183)	P16426	Epitope tags
AS21 4613	BAR \| Phosphinothricin N-acetyltransferase (36-50)	P16426	Epitope tags
AS22 4832	Ble tag \| Bleomycin resistance protein	P17493	Epitope tags
AS16 3690	Cas9 \| CRISPR-associated endonuclease 9 (polyclonal)	Q03JI6	Epitope tags
AS22 4855	CBD-Tag \| Chitin Binding Domain Epitope Tag		Epitope tags
AS21 4686	CBP epitope tag		Epitope tags
AS22 4854	CBP-tag \| Calmodulin Binding Protein Eptiope Tag		Epitope tags
AS21 4604	E Tag		Epitope tags
AS22 4800	GFP11 \| C-terminal of GFP	P42212	Epitope tags
AS21 4607	Glu-Glu Tag		Epitope tags
AS21 4605	Halo Tag		Epitope tags
AS21 4603	HSV epitope tag		Epitope tags
AS21 4681	HSV epitope tag		Epitope tags
AS21 4680	KT3 epitope tag		Epitope tags
AS21 4675	mStrawberry		Epitope tags
AS21 4602	Rho1D4/1D4 Tag	Epitope tag	Epitope tags
AS21 4684	S epitope tag	does not apply	Epitope tags
AS21 4606	SNAP/Clip Tag		Epitope tags
AS21 4508	Trx Tag \| Thioredoxin 1 Fusion protein		Epitope tags

Contact us with your antibody choice!

Agrisera Best Talk Prizes at the 32nd Western Photosynthesis Conference

Agrisera supported the 32nd Western Photosynthesis Conference, held between the 28th and 30th of April 2023, in Bodega Biological Marine Laboratory, California, USA.

The meeting covered the following topics:

Structure-Function Relations in Photosynthesis
Electron Transfer and Organization of Photosynthetic Complexes
Applied/Artificial Photosynthesis and Bioenergy
Global Ecology and Photosynthetic Response to the Dynamic Environment
Genomes and Evolution of Photosynthesis
Carbon Fixation and Central Carbon Metabolism

The winners of Agrisera Best Talk Prizes, and a free antibody of their choice from the Agrisera catalog, were:

Dr. Asmit Bhowmick from the Lawrence Berkeley National Laboratory, and Grad student Albert Liu from University of California Davis.

Awaiting your free antibody choices!

Awardess of Agrisera best talk prize at WPC2023

Convenient sets of antibodies for Arabidopsis research

For your convenience, chosen Agrisera antibodies can be purchased as sets at very favorabe pricing. The sets also include high-titer matching secondary antibodies, which can be used at 1: 25 000 1h/RT incubation, and Agrisera's ECL reagents, which offer versatile detection.

Agrisera offers the following antibody sets:

Educational antibody set for photosynthesis | Photosynthesis Tool kit – quantitation (includes standards for quantification of PSI and PSII) | Oxidative stress antibody set | Argonaute antibody set | Rubisco quantitation set (for Western blot or ELISA) | Plant Cell Compartment Antibody Marker Set for 3, 5 or 10 marker antibodies of your choice.

You are always welcome to suggest a new antibody set to us!

An example of Agrisera Antibody Set, including high-titer matching secondary antibodies and ECL reagent.

Latest

20 % off for antibodies to proteins involved in electron transfer in photosynthesis
2024-04-26 Get new tips and tricks for Western blot during Agrisera workshop on 16th of May at 11 am CET
2024-04-25 Detect and quantify Rubisco using antibodies with 20 years of publication record
2024-04-19 Which secondary antibody to choose for Western blot for protein detection in plant and algal samples?
2024-04-17 Study Photosystem I with Agrisera comprehensive antibody set
2024-04-14 10% discount in return for feedback
2024-04-12 Agrisera supports Eastern Regional Photosynthesis Conference 2024
2024-04-12 Most published photosynthetic antibodies, 20 % off until end of June 2024
2024-04-05 Agrisera online Western blot workshop for researchers in Taiwan, part I
2024-04-03 Versatile ECL reagent set, which can save your day!
2024-03-28

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