Alpha-mannosidase

AS09 452 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Canavalia ensiformis (Jack beans)

Product Information

Immunogen

native alpha-mannosidase purified from jack beans

Host Rabbit

Clonality Polyclonal

Purity Purified IgG

Format Lyophilized in phosphate buffer saline

Quantity 10 mg

Reconstitution For reconstitution add 1 ml of sterile destilled water.

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)

Reactivity

Confirmed reactivity Canavalia ensiformis (common jack-bean)

Not reactive in

Arabidopsis thaliana

Application examples

Additional information

Additional information Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.