Alpha-mannosidase

AS09 452 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Canavalia ensiformis (Jack beans)

Product Information

Immunogen

native alpha-mannosidase purified from jack beans

Host Rabbit

Clonality Polyclonal

Purity Purified IgG in PBS.

Format Lyophilized

Quantity 10 mg

Reconstitution For reconstitution add 1 ml of sterile destilled water

Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Tested applications ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)

Recommended dilution 1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)

Reactivity

Confirmed reactivity Canavalia ensiformis (common jack-bean)

Not reactive in

Arabidopsis thaliana

Application examples

Additional information

Additional information Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition

The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography

Background

Alpha-mannosidase cleaves the alpha form of mannose.

Product citations

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Confirmed reactivity:	Canavalia ensiformis (common jack-bean)
not reactive in:	Arabidopsis thaliana
background:	Alpha-mannosidase cleaves the alpha form of mannose.
additional information:	Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition
additional information (application):	The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography
Clonality:	Polyclonal
Format:	Lyophilized
Host:	Rabbit
immunogen:	native alpha-mannosidase purified from jack beans
Purity:	Purified IgG in PBS.
Quantity:	10 mg
recommended dilution:	1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
Reconstitution:	For reconstitution add 1 ml of sterile destilled water
storage:	Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications:	ELISA (ELISA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)