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Anti-SDH1/2 | Succinate dehydrogenase 1/2
AS23 4960 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Nicotiana tabacum, Solanum tuberosum, Vigna radiata
- Product Info
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Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana SDH1-1: UniProt: A0A178UAN3 TAIR: AT5G66760 and SDH1-2: UniProt: A0A178VRF6 TAIR: AT2G18450 Host: Rabbit Clonality: Polyclonal Purity: Antigen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution, add 50 µl of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Western blot (WB) Recommended dilution: 1 : 1000 - 1: 2000 (WB) Expected | apparent MW: 69.9 kDa - Reactivity
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Confirmed reactivity: Nicotiana tabacum (BY-2), Solanum tuberosum, Vigna radiata Predicted reactivity: Arabidopsis thaliana, Arachis hypogaea, Brachypodium distachyon, Brassica napus, Capsicum annuum, Cannabis sativa, Glycine max, Gossypium sp., Hordeum vulgare, Malus domestica, Manihot esculenta, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Pisum sativum, Populus sp., Solanum lycopersicum, Sorghum bicolor, Spinacia oleracea, Solanumtuberosum, Theobroma cacao, Triticum sp., Vitis vinifera, Zea mays
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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10 ug of mitochondrial protein from Nicotiana tabacum cell suspension (1A) BY-2 control cells, (2A) BY-2 2h stressed cells with 450 mM Mannitol, (3A) BY-2 2h stressed cells with 190 mM Mannitol, (M) PageRulerTM Plus Prestained Protein Ladder and 20 ug of mitochondrial protein from (1B) BY-2 control cells, (2B) BY-2 2h stressed cells with 450 mM Mannitol, (3B) BY-2 2h stressed cells with 190 mM Mannitol. Primary antibody was used in 1: 20 000 dilution. Secondary SDH ½ (Succinate dehydrogenase) antibody was used in 1:2000 dilution. Detection was performed using chemiluminescence by Bio- Rad ChemiDoc Imager, following manufacture’s recommendations.
- Background
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Background: SDH (Succinate dehydrogenase) is an enzyme complex found in the mitochondria. It plays a crucial role in both the citric acid cycle and the electron transport chain. SDH is composed of multiple subunits, typically referred to as SDH1, SDH2, SDH3, and SDH4. SDH1 is the flavoprotein subunit that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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Accessories
AS09 602 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)