Anti-TIM10| Mitochondrial import inner membrane translocase
AS23 4950 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana, Pisum sativum
- Data sheet
- Anti-TIM10| Mitochondrial import inner membrane translocase subunit TIM10
- Product Info
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Immunogen: KLH-conjugated, unique peptide derived from Arabidopsis thaliana TIM10 protein sequence. UniProt: Q9ZW33
TAIR: AT2G29530Host: Rabbit Clonality: Polyclonal Purity: Antigen affinity purified serum, in PBS pH 7.4 Format: Lyophilized Quantity: 50 µg Reconstitution: For reconstitution, add 50 µl of sterile or deionized water. Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. Tested applications: Western blot (WB) Recommended dilution: 1: 500 - 1 : 2000 (WB) Expected | apparent MW: 9.3 | 11 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana, Pisum sativum Predicted reactivity: Carica papaya, Brassica rapa, Eutrema salsugineum, Zea mays
Species of your interest not listed? Contact usNot reactive in: No confirmed exceptions from predicted reactivity are currently known - Application Examples
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Samples:
1 - 50 ug of Arabidopsis thaliana seedling isolated mitochondria extract #1
2 - 50 ug of Arabidopsis thaliana seedling isolated mitochondria extract #2
3 - 50 ug of Arabidopsis thaliana cell culture isolated mitochondria extract
4 - 50 ug of Pisum sativum seedling isolated mitochondria extract 5 - 50 ug of Oryza sativa seedling isolated mitochondria extract Mark: Blue Protein Standard MW markers
50 µg/well of total protein extracted freshly from the seedlings of Arabidopsis thaliana, Pisum sativum and Oryza sativa. The mitochondria were extracted according to Murcha and Whelan (2015) mitochondria isolation protocol pages 1-12. Samples were separated in the cold on 14 % SDS-PAGE and blotted for 1 h to nitrocellulose (pore size of 0.45 um), using semi-dry method in the cold. Blot was blocked with 10 % skim milk for: 4°C/ON with agitation. Blot was incubated in the primary antibody at a dilution of 1: 500 in 1X TBS-Tween for ON/4°C with agitation. The antibody solution was decanted, and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1: 10 000 in 1X TBS-Tween for 1h/RT with agitation. The blot was washed as above and developed with a following chemiluminescent detection reagent. Exposure time was 10 minutes.
Mitochondria were extracted according to the method described here: MURCHA, M. W. & WHELAN, J. 2015. Isolation of Intact Mitochondria from the Model Plant Species Arabidopsis thaliana and Oryza sativa. In: WHELAN, J. & MURCHA, M. W. (eds.) Plant Mitochondria: Methods and Protocols. New York, NY: Springer New York.
Courtesy of Valencia Marisa, The University of Western Australia, Australia - Background
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Background: TIM10 (Mitochondrial import inner membrane translocase subunit TIM10) acts as intermembrane chaperone that participates in the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Poster Collection - Reviews:
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