Anti-Arabinogalactan-Protein, AGP (monoclonal, clone JIM4)

Product no: AS22 4743-1ml
AS22 4743-1ml  | Clonality: Monoclonal  |  Host: Rat  |  Reactivity: Higher plants

From the laboratory of Paul Knox, Phd, University of Leeds, United Kingdom
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  • Product Info
  • Immunogen: Polysaccharide Arabinogalactan-protein (AGP) from Oryza sativa
    Sub class: IgM
    Host: Rat
    Clonality: Monoclonal
    Purity: Cell Culture Supernatant, with 0.05% sodium azide.
    Format: Liquid
    Quantity: 1 ml
    Storage: Store at -20°C. Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
    Tested applications: Immunofluorescence (IF)
    Recommended dilution: 1: 10 (IF)
  • Reactivity
  • Confirmed reactivity: Higher plants
  • Additional Information
  • Additional information (application): Antibody is recognizing carbohydrate epitope containing Β-linked glucuronic acid.
  • Background
  • Background: The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Arabinogalactans can be found in plants as free glycans, or attached to rhamnogalacturonan-I or protein backbones.
  • Protocols
  • Immunofluorsecence as described in: Developmental complexity of arabinan polysaccharides and their processing in plant cell walls Verhertbruggen Y, Marcus SE, Haeger A, Verhoef R, Schols HA, McCleary BV, McKee L, Gilbert HJ, Knox JP., The Plant Journal (2009) 59, 413–425.
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