Anti-ATG9 | Autophagy-related protein 9 (N-terminal)

Product no: AS16 4071

AS16 4071   | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana ATG9 protein sequence, N-terminal part, UniProt: Q8RUS5, TAIR: AT2G31260

    Host: Rabbit
    Clonality: Polyclonal
    Purity: Immunogen affinity purified serum in PBS pH 7.4.
    Format: Lyophilized
    Quantity: 50 µg
    Reconstitution: For reconstitution add 50 µl of sterile water
    Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution:

    1 : 10 000 (WB)

    Expected | apparent MW: 99.5 | 100 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Brassica campestris, Brassica rapa subsp. pekinensis, Capsella rubella, Eutrema salsugineum

    Species of your interest not listed? Contact us
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples

  • western blot using anti-ATG9 antibodies

    Increasing amount of microsomal fraction of Arabidopsis thaliana cell culture (2,3,4).  Microsomal protein was extracted accoring to Maudoux et al. (2000).   and denatured with loading buffer (2 % SDS) at 37°C for 1h before SDS-PAGE gel electrophoresis and blotted to PVDF using semi-dry. Blots were blocked with 3 % nonfat milk + 0.1 % goat serum in TBS-T (Tween 20)  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 + 1 % goat serum for ON at 4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:10 000 in TBS-T + 1 % goat serum for 1h at RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent. Exposure time was  seconds.
    Red marker band has apparent mass of 72 kDa. 

    Courtesy of Dr. Henri Batoko, University of Louvain, Belgium 

  • Background
  • Background: ATG9 (Autophagy-related protein 9) is required for autophagy that plays an essential role in plant nutrient recycling. 
    Alternative name: AtAPG9.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Oxygenic photosynthesis poster by prof. Govindjee and Dr. Shevela

    Z-scheme of photosynthetic electron transport by prof. Govindjee and Dr. Björn and Dr. Shevela
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AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

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AS10 1547 | Host: Goat

For larger volumes, please inquire
147 €
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AS16 4072   | Clonality: Polyclonal  |  Host: Rabbit | Reactivity: Arabidopsis thaliana

319 €
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Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
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AS16 ECL-S | extreme low femtogram detection  |  Limited stock

This product can be purchased in 3 different volumes:

AS16 ECL-S-10, 10 ml. Trial size limited to one per customer

AS16 ECL-S-100, 100 ml

Choose the appropriate volume in the drop down menu to the right

From 17 €