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Anti-ECA1 | Calcium-transporting ATPase 1, endoplasmic reticulum-type
Product no: AS17 4156
AS17 4156 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
329
€
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- Product Info
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Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana
ECA1 sequence, cytosolic loop, between TM4 and TM5, UniProt: P92939, TAIR: At1g07810Host: Rabbit Clonality: Polyclonal Purity: Serum Format: Lyophilized Quantity: 50 µl Reconstitution: For reconstitution add 50 µl of sterile water Storage: Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Tested applications: Western blot (WB) Recommended dilution: 1 : 10 000 (WB) Expected | apparent MW: 116.3 kDa - Reactivity
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Confirmed reactivity: Arabidopsis thaliana Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information (application): The protein shows tissue specific expression, shown on a blot included in Wu et al. (2000). Lowest amounts of ECA1 are found in siliques. - Background
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Background: Calcium-transporting ATPase 1, endoplasmic reticulum-type is a magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the endoplasmic reticulum lumen. Also regulate manganese ion homeostasis by pumping it into endomembrane compartments. The enzyme can also transport zinc. Alternative names: ACA3, ARABIDOPSIS THALIANA ER-TYPE CA2+-ATPASE 1, ATECA1, ECA1, ER-TYPE CA2+-ATPASE 1. - Product Citations
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Selected references: Wu et al. (2000). An endoplasmic reticulum-bound Ca(2+)/Mn(2+) pump, ECA1, supports plant growth and confers tolerance to Mn(2+) stress. Plant Physiol. 2002 Sep;130(1):128-37.
Liang et al. (1997). ECA1 complements yeast mutants defective in Ca2+ pumps and encodes an endoplasmic reticulum-type Ca2+-ATPase in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 1997 Aug 5;94(16):8579-84. - Protocols
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Agrisera Western Blot protocol and video tutorials
Protocols to work with plant and algal protein extracts
Agrisera Educational Posters Collection - Reviews:
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AS17 4156 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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