ECA1 | Calcium-transporting ATPase 1, endoplasmic reticulum-type

Product no: AS17 4156

AS17 4156 | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana
    ECA1 sequence, cytosolic loop, between TM4 and TM5, UniProt:P92939, TAIR: At1g07810
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l, of sterile water
    Storage: Lyophilized antibody can be stored at -20C for up to 3 years. Re-constituted antibody can be stored at 4C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 10 000 (WB)
    Expected | apparent MW: 116,3 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Additional Information
  • Additional information (application): The protein shows tissue specific expression, shown on a blot included in Wu et al. (2000). Lowest amounts of ECA1 are found in siliques.
  • Background
  • Background: Calcium-transporting ATPase 1, endoplasmic reticulum-type is  a magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the endoplasmic reticulum lumen. Also regulate manganese ion homeostasis by pumping it into endomembrane compartments. The enzyme can also transport zinc. Alternative names: ACA3, ARABIDOPSIS THALIANA ER-TYPE CA2+-ATPASE 1, ATECA1, ECA1, ER-TYPE CA2+-ATPASE 1.
  • Product Citations
  • Selected references: Wu et al. (2000). An endoplasmic reticulum-bound Ca(2+)/Mn(2+) pump, ECA1, supports plant growth and confers tolerance to Mn(2+) stress. Plant Physiol. 2002 Sep;130(1):128-37.
    Liang et al. (1997). ECA1 complements yeast mutants defective in Ca2+ pumps and encodes an endoplasmic reticulum-type Ca2+-ATPase in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 1997 Aug 5;94(16):8579-84.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Posters Collection
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AS17 4156 | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana

319 €
Add to cart
Info
Buy 2 items of this product for 239.00 €/items
Buy 3 items of this product for 217.00 €/items
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