Name:
Phone:
E-mail:
Address:






Deg1 | chloroplastic DegP-type serine protease 1

AS07 246 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, P. sativum, Z. mays
Deg1 | chloroplastic DegP-type serine protease 1 in the group Antibodies Plant/Algal  / Photosynthesis  / Proteases at Agrisera AB (Antibodies for research) (AS07 246)
Deg1 | chloroplastic DegP-type serine protease 1



DATA SHEET IN PDF

Qty: 
394
How to cite this product:
Product name, number (Agrisera, Sweden)

Data sheet Product citations Add review

Product Information

Immunogen

Recombinant Deg1 of Arabidopsis thaliana O22609, At3g27925, overexpressed in E.coli

Host Rabbit
Clonality Polyclonal
Purity Total IgG. Protein G purified in PBS pH 7.4.
Format Lyophilized
Quantity 360 ĩg
Reconstitution For reconstitution add 200 ĩl of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 500/5 ug thylakoid fraction (WB)
Expected | apparent MW 46,6 | 36 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana, Pisum sativum, Zea mays
Predicted reactivity Ricinus communis
Species of your interest not listed? Contact us
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples

Application example

 

Western blot using anti-Deg1 antibodies

(1) Pisum sativum thylakoid fraction, (2) Arabidopsis thaliana thylakoid fraction, (3) recombinant Deg1 expressed in E.coli. Primary antibody has been used at 1: 500 dilution with chemiluminescent detection reagent.

Additional information

Unspecific reaction at ca, 30 kDa and ca, 85 kDa (checked by by mass spec, and confirmed not to be neither Deg1 nor any other protease, The band at the right MW does contain Deg1 by our MS analysis

Related products

Background

Background

Deg1 belongs to S1 chymotrypsin protease family and contains 16 protein members in Arabidopsis thaliana. Deg1 is localized to chloroplast.

Product citations

Selected references Zienkiewicz et al. (2013). Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plant Physiol. & Biochem. March 16.
immunogen:

Recombinant Deg1 of Arabidopsis thaliana O22609, At3g27925, overexpressed in E.coli

Host: Rabbit
Clonality: Polyclonal
Purity: Total IgG. Protein G purified in PBS pH 7.4.
Format: Lyophilized
Quantity: 360 ĩg
Reconstitution: For reconstitution add 200 ĩl of sterile water
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications: Western blot (WB)
recommended dilution: 1 : 500/5 ug thylakoid fraction (WB)
Expected | apparent MW: 46,6 | 36 kDa
Confirmed reactivity: Arabidopsis thaliana, Pisum sativum, Zea mays
predicted reactivity: Ricinus communis
Species of your interest not listed? Contact us
not reactive in: No confirmed exceptions from predicted reactivity are currently known
Picture (footer):

Application example

 

Western blot using anti-Deg1 antibodies

(1) Pisum sativum thylakoid fraction, (2) Arabidopsis thaliana thylakoid fraction, (3) recombinant Deg1 expressed in E.coli. Primary antibody has been used at 1: 500 dilution with chemiluminescent detection reagent.

additional information (application): Unspecific reaction at ca, 30 kDa and ca, 85 kDa (checked by by mass spec, and confirmed not to be neither Deg1 nor any other protease, The band at the right MW does contain Deg1 by our MS analysis
background:

Deg1 belongs to S1 chymotrypsin protease family and contains 16 protein members in Arabidopsis thaliana. Deg1 is localized to chloroplast.

All references: Zienkiewicz et al. (2013). Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plant Physiol. & Biochem. March 16.

Related products: Deg1 | chloroplastic DegP-type serine protease 1

AS16 ECL-S-N | low pico to mid femtogram and extreme low femtogram detection

Now available only...
From 26 €
AS10 1581 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
407 €
Buy 2 items of this product for 330.00 €/items
Buy 3 items of this product for 218.00 €/items
AS09 602 |  Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)

10 ...
201 €