Deg1 | chloroplastic DegP-type serine protease 1
AS07 246 | Clonality: Polyclonal | Host: Rabbit | Reactivity: A. thaliana, P. sativum, Z. mays
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Product Information
Host
Rabbit
Clonality
Polyclonal
Purity
Total IgG
Format
Lyophilized in PBS pH 7.4
Quantity
360 µg
Reconstitution
For reconstitution add 200 µl of sterile water.
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Tested applications
Western blot (WB)
Recommended dilution
1 : 500/5 ug thylakoid fraction (WB)
Expected | apparent MW
46.6 | 36 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana, Pisum sativum, Zea mays
Predicted reactivity
Ricinus communis
Not reactive in
No confirmed exceptions from predicted reactivity are currently known.
Application examples
Application examples
Application example
(1) Pisum sativum thylakoid fraction, (2) Arabidopsis thaliana thylakoid fraction, (3) recombinant Deg1 expressed in E.coli. Primary antibody has been used at 1: 500 dilution with chemiluminescent detection reagent.
Additional information
Unspecific reaction at ca. 30 kDa and ca. 85 kDa (checked by by mass spec, and confirmed not to be neither Deg1 nor any other protease. The band at the right MW does contain Deg1 by our MS analysis.
Background
Background
Deg1 belongs to S1 chymotrypsin protease family and contains 16 protein members in Arabidopsis thaliana. Deg1 is localized to chloroplast.
Product citations
Selected references
Zienkiewicz et al. (2013). Light intensity and quality stimulated Deg1-dependent cleavage of PSII components in the chloroplasts of maize. Plant Physiol. & Biochem. March 16.
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