DEGP12 | Putative protease Do-like 12 (mitochondrial)
AS19 4345 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana

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Product Information
Reactivity
Application examples

40, 20 and 10 µg of recombinant Arabidopsis thaliana DEG12 were separated on 8 % SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk for 2h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 3.4 µg/ml in PBS-T 1h/RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 10 min. in PBS-T at RT with agitation. Blot was incubated in the matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, following manufacture's instructions.
Additional information
Reactivit of this antibody on endogenous material remains to be determined.
Background
Alternative names: Degradation of Periplasmic Proteins 12.
Product citations
Related products: DEGP12 | Putative protease Do-like 12 (mitochondrial)
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