Anti-DEGP12 | Putative protease Do-like 12 (mitochondrial)

Product no: AS19 4345

AS19 4345 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

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Delivery:  3-6 business days
  • Product Info
  • Immunogen: Recombinant Putative protease Do-like 12 derived from the sequence of Arabidopsis thaliana, amino acids 25-499, UniProt: Q9LK70 TAIR: AT3G16550
    Host: Rabbit
    Clonality: Polyclonal
    Purity: Total IgG. Protein G purified.
    Format: Liquid
    Quantity: 50 g
    Storage: Store at -20C or -80C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: ELISA (ELISA), Western blot (WB)
    Recommended dilution: 1 : 1000 - 1: 2000 (WB)
    Expected | apparent MW: 54 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • application example

    western blot using anti-Arabidopsis thaliana DEGP12 antibodies

    40, 20 and 10 µg of recombinant Arabidopsis thaliana DEG12 were separated on 8 % SDS-PAGE  and blotted 1h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk for 2h/RT  with agitation. Blot was incubated in the primary antibody at a dilution of 3.4 µg/ml in PBS-T 1h/RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 10 min. in PBS-T at RT with agitation. Blot was incubated in the matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in  for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, following manufacture's instructions. 

  • Additional Information
  • Additional information: Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4

    Reactivit of this antibody on endogenous material remains to be determined. 
  • Background
  • Background: Putative protease Do-like 12 is a mitochondrial enzyme with serine-type endopeptidase avtivity (EC 3.4.21.-) involved in proteolysis. Localized to mitochondrial matrix. 
    Alternative names: Degradation of Periplasmic Proteins 12.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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