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DEGP12 | Putative protease Do-like 12 (mitochondrial)

AS19 4345 | Clonality: Polyclonal  |  Host: Rabbit  | Reactivity: Arabidopsis thaliana

DEGP12 | Putative protease Do-like 12 (mitochondrial) in the group Antibodies for Plant/Algal  / Mitochondria | Respiration at Agrisera AB (Antibodies for research) (AS19 4345)

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Product Information

Immunogen Recombinant Putative protease Do-like 12 derived from the sequence of Arabidopsis thaliana, amino acids 25-499, UniProt: Q9LK70 TAIR: AT3G16550
Host Rabbit
Clonality Polyclonal
Purity >95%, Protein G purified, to a total immunoglobulin G fraction
Format Liquid
Quantity 50 ug
Storage Store at -20°C or -80°C, avoid repeated freeze-thaw cycles. Make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications ELISA (ELISA), Western blot (WB)
Recommended dilution 1 : 1000 - 1: 2000 (WB)
Expected | apparent MW 54 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples application example


western blot using anti-Arabidopsis thaliana DEGP12 antibodies


40, 20 and 10 µg of recombinant Arabidopsis thaliana DEG12 were separated on 8 % SDS-PAGE  and blotted 1h to PVDF using semi-dry transfer. Blot was blocked with 5 % milk for 2h/RT  with agitation. Blot was incubated in the primary antibody at a dilution of 3.4 µg/ml in PBS-T 1h/RT with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed 4 times for 10 min. in PBS-T at RT with agitation. Blot was incubated in the matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated) diluted to 1:50 000 in  for 1h/RT with agitation. The blot was washed as above and developed with chemiluminescent detection reagent, following manufacture's instructions. 

Additional information

Additional information Preservative: 0.03% Proclin 300. Preparation contains: 50% Glycerol, 10 mM PBS, pH 7.4

Reactivit of this antibody on endogenous material remains to be determined. 

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Plant and algal protein extraction buffer

Background

Background Putative protease Do-like 12 is a mitochondrial enzyme with serine-type endopeptidase avtivity (EC 3.4.21.-) involved in proteolysis. Localized to mitochondrial matrix. 
Alternative names: Degradation of Periplasmic Proteins 12.

Product citations

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