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Donkey anti-Mouse IgG (H&L), F(ab)'2 fragment, ALP conjugate, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG

AS16 3439 | Clonality: Polyclonal | Host: Donkey | Reactivity: Mouse IgG (H&L), F(ab)'2 Fragment
Donkey anti-Mouse IgG (H&L), F(ab)'2 fragment, ALP conjugate, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG in the group Secondary Antibodies / Anti-Mouse / ALP (alkaline phosphatase) at Agrisera AB (Antibodies for research) (AS16 3439)

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Product Information

Immunogen Purified Mouse IgG, whole molecule
Host Donkey
Clonality Polyclonal
Purity Affinity purified
Format Liquid
Quantity 0.5 mg
Storage Store non-diluted antibody at Store at 2-8°C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.
Tested applications Flow cytometry (Flow cyt), Immunolocalization (IL)
Recommended dilution 1 : 20-1 : 2000 (Flow cyt), (IL)

Reactivity

Confirmed reactivity Mouse IgG (H&L)

Application examples

Additional information

Additional information

Antibody purity is > 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Affinity purified using solid phase Mouse IgG .
Antibody is supplied in 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free. 0.05% (w/v) Sodium Azide is added as a preservative.

Based on IEP, this antibody reacts with: • heavy (γ) chains on mouse IgG • light chains on all mouse immunoglobulins .
Based on IEP, no reactivity is observed to: • non-immunoglobulin mouse serum immunoglobulins • IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep .

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Background

Background

Donkey anti-Mouse IgG (H&L), F(ab)'2 fragment, ALP conjugate, min. cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG is a secondary antibody conjugated to ALP, which binds to mouse IgG (H&L), F(ab)'2 Fragment in immunological assays.

Product citations

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