ERD7 | Early Response to Dehydration 7

Product no: AS19 4317

AS19 4317  | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana

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  • Product Info
  • Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana ERD7, UniProt: O48832 TAIR: AT2G17840
    Host:

    Rabbit

    Clonality: Polyclonal
    Purity: Serum
    Format: Lyophilized
    Quantity: 50 l
    Reconstitution: For reconstitution add 50 l, of sterile water
    Storage: Store lyophilized/reconstituted at -20C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
    Tested applications: Western blot (WB)
    Recommended dilution: 1 : 2000 (WB)
    Expected | apparent MW: 49 | 58 kDa
  • Reactivity
  • Confirmed reactivity: Arabidopsis thaliana
    Predicted reactivity: Arabidopsis thaliana
    Not reactive in: No confirmed exceptions from predicted reactivity are currently known
  • Application Examples
  • Western blot using anti-ERD7 antibodies

    Ten µg of total protein extracted freshly from Arabidopsis thaliana total leaf  with protein extraction buffer (Tris-HCl 50 mM pH 8.0, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 0.5% Na-Deoxycholate, 2 mM PMSF, 2 mM DTT) and denatured with Laemmli sample buffer containing 2% beta-mercaptoethanol at 70°C for 10 min. Samples were separated on 10% SDS-PAGE  and blotted 1h to PVDF using  semi-dry  transfer. Blot was blocked with 5% non-fat milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 in TBS-T with 1% milk for ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5-10 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:25 000 in TBS-T with 1% milk for 1h/RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent according to manufacture's instructions.  Exposure time was 1 min.

    Courtesty Dr. Hiroaki Fujii, University of Turku, Finland







  • Additional Information
  • Additional information (application): To induce detectable levels of ERD7,  plants need to be exposed to low temperature of 4 ºC for 24 h.

    The protein is detected in the microsomal fraction. (Barajas-Lopez et al, 2021)
  • Background
  • Background: ERD7 (Early Response to Dehydration ) and its homologs are important for plant stress responses and development and associated with modification of membrane lipid composition. 
  • Product Citations
  • Selected references: Barajas-Lopez et al. (2021) EARLY RESPONSE TO DEHYDRATION 7 Remodels Cell Membrane Lipid Composition during Cold Stress in Arabidopsis. Plant Cell Physiol. 2021 Mar 25;62(1):80-91. doi: 10.1093/pcp/pcaa139. PMID: 33165601.
  • Protocols
  • Agrisera Western Blot protocol and video tutorials

    Protocols to work with plant and algal protein extracts

    Agrisera Educational Poster Collection
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