ERD7 | Early Response to Dehydration 7
AS19 4317 | Clonality: Polyclonal | Host: Rabbit | Reactivity: Arabidopsis thaliana
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Product Information
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana ERD7, UniProt: O48832 TAIR: AT2G17840
Host
Rabbit
Clonality
Polyclonal
Purity
Serum
Format
Lyophilized
Quantity
50 ĩl
Reconstitution
For reconstitution add 50 ĩl, of sterile water
Storage
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications
Western blot (WB)
Recommended dilution
1 : 2000 (WB)
Expected | apparent MW
49 | 58 kDa
Reactivity
Confirmed reactivity
Arabidopsis thaliana
Predicted reactivity
Arabidopsis thaliana
Not reactive in
No confirmed exceptions from predicted reactivity are currently known
Application examples
Application examples
Ten µg of total protein extracted freshly from Arabidopsis thaliana total leaf with protein extraction buffer (Tris-HCl 50 mM pH 8.0, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 0.5% Na-Deoxycholate, 2 mM PMSF, 2 mM DTT) and denatured with Laemmli sample buffer containing 2% beta-mercaptoethanol at 70°C for 10 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blot was blocked with 5% non-fat milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 in TBS-T with 1% milk for ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5-10 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:25 000 in TBS-T with 1% milk for 1h/RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent according to manufacture's instructions. Exposure time was 1 min.
Courtesty Dr. Hiroaki Fujii, University of Turku, Finland
Ten µg of total protein extracted freshly from Arabidopsis thaliana total leaf with protein extraction buffer (Tris-HCl 50 mM pH 8.0, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 0.5% Na-Deoxycholate, 2 mM PMSF, 2 mM DTT) and denatured with Laemmli sample buffer containing 2% beta-mercaptoethanol at 70°C for 10 min. Samples were separated on 10% SDS-PAGE and blotted 1h to PVDF using semi-dry transfer. Blot was blocked with 5% non-fat milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 in TBS-T with 1% milk for ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5-10 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:25 000 in TBS-T with 1% milk for 1h/RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent according to manufacture's instructions. Exposure time was 1 min.
Courtesty Dr. Hiroaki Fujii, University of Turku, Finland
Additional information
To induce detectable levels of ERD7, plants need to be exposed to low temperature of 4 ºC for 24 h.
The protein is detected in the microsomal fraction. (Barajas-Lopez et al, 2021)
The protein is detected in the microsomal fraction. (Barajas-Lopez et al, 2021)
Background
Background
ERD7 (Early Response to Dehydration ) and its homologs are important for plant stress responses and development and associated with modification of membrane lipid composition.
Product citations
Selected references
Barajas-Lopez et al. (2021) EARLY RESPONSE TO DEHYDRATION 7 Remodels Cell Membrane Lipid Composition during Cold Stress in Arabidopsis. Plant Cell Physiol. 2021 Mar 25;62(1):80-91. doi: 10.1093/pcp/pcaa139. PMID: 33165601.
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