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ERD7 | Early Response to Dehydration 7

AS19 4317  | Clonality: Polyclonal |  Host: Rabbit | Reactivity: Arabidopsis thaliana

ERD7 | Early Response to Dehydration 7   in the group Antibodies Plant/Algal  / Environmental Stress / Cold stress at Agrisera AB (Antibodies for research) (AS19 4317)
ERD7 | Early Response to Dehydration 7



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Product Information

Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana ERD7, UniProt: O48832 TAIR: AT2G17840
Host

Rabbit

Clonality Polyclonal
Purity Serum
Format Lyophilized
Quantity 50 ĩl
Reconstitution For reconstitution add 50 ĩl, of sterile water
Storage Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Tested applications Western blot (WB)
Recommended dilution 1 : 2000 (WB)
Expected | apparent MW 49 | 58 kDa

Reactivity

Confirmed reactivity Arabidopsis thaliana
Predicted reactivity Arabidopsis thaliana
Not reactive in No confirmed exceptions from predicted reactivity are currently known

Application examples

Application examples Western blot using anti-ERD7 antibodies

Ten µg of total protein extracted freshly from Arabidopsis thaliana total leaf  with protein extraction buffer (Tris-HCl 50 mM pH 8.0, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 0.5% Na-Deoxycholate, 2 mM PMSF, 2 mM DTT) and denatured with Laemmli sample buffer containing 2% beta-mercaptoethanol at 70°C for 10 min. Samples were separated on 10% SDS-PAGE  and blotted 1h to PVDF using  semi-dry  transfer. Blot was blocked with 5% non-fat milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 in TBS-T with 1% milk for ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5-10 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:25 000 in TBS-T with 1% milk for 1h/RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent according to manufacture's instructions.  Exposure time was 1 min.

Courtesty Dr. Hiroaki Fujii, University of Turku, Finland







Additional information

To induce detectable levels of ERD7,  plants need to be exposed to low temperature of 4 ºC for 24 h.

The protein is detected in the microsomal fraction. (Barajas-Lopez et al, 2021)

Related products

Background

Background ERD7 (Early Response to Dehydration ) and its homologs are important for plant stress responses and development and associated with modification of membrane lipid composition. 

Product citations

Selected references Barajas-Lopez et al. (2021) EARLY RESPONSE TO DEHYDRATION 7 Remodels Cell Membrane Lipid Composition during Cold Stress in Arabidopsis. Plant Cell Physiol. 2021 Mar 25;62(1):80-91. doi: 10.1093/pcp/pcaa139. PMID: 33165601.
immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana ERD7, UniProt: O48832 TAIR: AT2G17840
Reconstitution: For reconstitution add 50 ĩl, of sterile water
Host:

Rabbit

Clonality: Polyclonal
Purity: Serum
Format: Lyophilized
Quantity: 50 ĩl
storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
tested applications: Western blot (WB)
recommended dilution: 1 : 2000 (WB)
calculated | apparent molecular mass [kDa]: 49 | 58 kDa
Confirmed reactivity: Arabidopsis thaliana
predicted reactivity: Arabidopsis thaliana
not reactive in: No confirmed exceptions from predicted reactivity are currently known
Picture (footer): Western blot using anti-ERD7 antibodies

Ten µg of total protein extracted freshly from Arabidopsis thaliana total leaf  with protein extraction buffer (Tris-HCl 50 mM pH 8.0, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 0.5% Na-Deoxycholate, 2 mM PMSF, 2 mM DTT) and denatured with Laemmli sample buffer containing 2% beta-mercaptoethanol at 70°C for 10 min. Samples were separated on 10% SDS-PAGE  and blotted 1h to PVDF using  semi-dry  transfer. Blot was blocked with 5% non-fat milk for 1h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1: 2 000 in TBS-T with 1% milk for ON/4°C with agitation. The antibody solution was decanted and the blot was rinsed briefly, then washed 3 times for 5-10 min in TBS-T at RT with agitation. Blot was incubated in Agrisera matching secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated AS09 602) diluted to 1:25 000 in TBS-T with 1% milk for 1h/RT with agitation. The blot was washed as above and developed for 5 min with chemiluminescent detection reagent according to manufacture's instructions.  Exposure time was 1 min.

Courtesty Dr. Hiroaki Fujii, University of Turku, Finland







additional information (application): To induce detectable levels of ERD7,  plants need to be exposed to low temperature of 4 ºC for 24 h.

The protein is detected in the microsomal fraction. (Barajas-Lopez et al, 2021)
background: ERD7 (Early Response to Dehydration ) and its homologs are important for plant stress responses and development and associated with modification of membrane lipid composition. 
All references: Barajas-Lopez et al. (2021) EARLY RESPONSE TO DEHYDRATION 7 Remodels Cell Membrane Lipid Composition during Cold Stress in Arabidopsis. Plant Cell Physiol. 2021 Mar 25;62(1):80-91. doi: 10.1093/pcp/pcaa139. PMID: 33165601.

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